膀胱移行细胞癌肿瘤血管形成的定量试验

目的　探讨膀胱移行细胞癌 (TCCB)中微血管密度 (MVD)与肿瘤分期、分级及淋巴结状态等参数之间的关系。方法　应用免疫组织化学SP法对 3 5例TCCB中的内皮细胞进行染色 ,用MVD表示血管形成的程度。结果　TCCB组织中有淋巴结转移病人的MVD明显高于无转移组 (P <0 .0 1)。MVD与肿瘤分期、分级间有显著相关性。高分化癌、中分化癌的MVD明显低于低分化癌 (P <0 .0 1) ,肿瘤组织浸润至膀胱壁外 (T3b +4)病人其MVD高于局限于膀胱壁内 (Ta+1+2 +3a)者 (P <0 .0 5 )。结论　肿瘤血管形成在TCCB的生长转移过程中起到重要作用 ,MVD是衡量肿瘤血管形成程度的定量指标 ,是传统预后参数基础上能反映TCCB预后的一个重要指标。

Studies of microvessel density(MVD) in transitional cell carcinomas of bladder

Objective To evaluate the relationship between MVD and the established prognostic indicators(i.e.tumor stage,pathological grade and regional lymph node status)in transitional cell carcinoma of bladder(TCCB).Methods Tumor tissue was obtained from 35 patients with primary TCCB.Microvessels density was identified by immunohistochemical method using the antibody to factor VⅢ-related antigen,a rabbit monoclonal antibody directed against the endothelial cell antigen.Results Evaluation of 35 tumors revealed that MVD from the patients without lymph node metastasis were significantly lower than the ones with metastasis(P<0.01).MVD was associated with histologic grade(G 1+2 vs.G 3,P<0.01)and pathologic stage (T a+1+2+3a vs T 3b+4 ,P<0.05).Conclusion These data indicate tumor angiogenesis is essential in tumor growth and metastasis.MVD is an quantitive indicator in judging tumor angiogenesis,and it is helpful to reflecting the propnsis based on the established parameters.