Effect of bone marrow mesenchymal stem cells transfected with rAAV2-bFGF on early angiogenesis of calvarial defects in rats |
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Authors: | Meiling CHEN Ke SONG Nianjing RAO Mengqi HUANG Zhengjiang Huang Yingguang CAO |
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Institution: | Center of Stomatological Medicine,Tongii Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China |
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Abstract: | The purpose of this study was to evaluate the effect of bone marrow mesenchymal stem cells (MSCs) transfected with the basic
fibroblast growth factor (bFGF)-expressing recombinant adeno-associated virus vector (rAAV2-bFGF), on early angiogenesis of
calvarial defects in rats. The MSCs were cultured and transfected with rAAV2-bFGF after differential adherence isolation.
The transfection efficiency was detected by RT-PCR and Western blotting. The transfected MSCs were compounded with poly-DL-lactide/hydroxyapatite
(PDLLA/HA) in vitro. The cranial defect models in 36 male SD rats were created. Nothing (group A), PDLLA/HA alone (group B), PDLLA/HA combined
with MSCs (group C), and PDLLA/HA combined with rAAV2-bFGF transfected MSCs (group D) were implanted in rat calvarial defects.
The specimens were harvested for hematoxylin-eosin staining on the day 1, 3 and 7 after implantation. Factor VIII immunohistochemical
staining and histomorphometric analysis were carried out to evaluate neovascularization around the implantation. The results
indicated that MSCs could indeed be successfully transfected with the rAAV2-bFGF vector. Histological and histomorphometric
analysis revealed that the angiogenesis in group D was significantly enhanced as compared with the rest groups (P<0.05). These results strongly suggest that MSCs transfected with rAAV2-bFGF in combination with PDLLA/HA can effectively
promote the early angiogenesis of calvarial defects in rats, which played an important role in creating an environment suitable
for the survival and activity of transplanted cells for further applications in cranio-maxillofacial bone regeneration. |
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Keywords: | basic fibroblast growth factor gene therapy angiogenesis bone regeneration |
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