高滴度天然抗角蛋白自身抗体转基因小鼠模型的建立

目的 建立天然抗角蛋白自身抗体(AK auto Ab)的转基因小鼠模型。方法 将转基因表达质粒显微注射法转入CBA×C57BL/6小鼠受精卵,导入假孕雌鼠输卵管后培育子代小鼠;PCR鉴定子代小鼠基因型;ELISA法分析转基因小鼠体内AK auto Ab水平的变化。结果 共获得12系转基因阳性的Founder小鼠,其中9系传至第3代;3系转基因小鼠体内的AK auto Ab水平显著增高。结论 成功建立AK auto Ab的转基因小鼠,获得了血清AKautoAb水平增高的动物模型。

Construction of a Transgenic Mice Model with High Serum Titer of Natural Anti-keratin Autoantibody

Objective To construct an anti-keratin autoantibody (AK auto Ab) transgenic mouse model. Methods Linearized transgene plasmid was microinjected into the zygotes of CBA×C57BL/6 mice, which were transplanted into the oviducts of pseudo-pregnant mice. PCR was used to identify the genotype of the offsprings, and ELISA was applied to measure the serum levels of AK auto Ab. Results Twelve transgene positive founder mice were obtained, and 9 of them produced offsprings as the third generation. The serum level of AK auto Ab was increased in 3 of the transgenic mice. Conclusions AK auto Ab transgenic mice were successfully established; these mice could serve as an animal model with increased serum levels of AK auto Ab.