| A Dual Wavelength Differential First Derivative Spectrophotometric Method for Identification and Determination of Carbon Monoxide Generated During the Microsomal Metabolism of Xenobiotics in vitro |
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| 引用本文： | 徐宏祥,刘志强. A Dual Wavelength Differential First Derivative Spectrophotometric Method for Identification and Determination of Carbon Monoxide Generated During the Microsomal Metabolism of Xenobiotics in vitro[J]. 中国药学, 1997, 0(2) |
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| 作者姓名： | 徐宏祥 刘志强 |
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| 作者单位： | 浙江嘉兴市药品检验所(徐宏祥)，浙江医科大学药学系(刘志强) |
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| 摘 要： | 本文研究了双波长差示一阶导数分光光度法运用于作为对照品的一氧化碳（ＣＯ）饱和水浓度标定及定性与定量测定微粒体代谢中生成的一氧化碳的方法。本法的优点在于能显著消除试样本底干扰，大大提高了定量准确性及灵敏度。在ＣＯ浓度２～１０μｍｏｌ·Ｌ１范围内与导数光谱峰（４１５ｎｍ）和谷（４２６ｎｍ）之间距离（Ｄ）呈良好线性关系，ｒ＝０．９９９９（ｎ＝５），回归方程Ｃ（ｍｍｏｌ·Ｌ１）＝１７．６Ｄ０．４。最低检测浓度低于０．１μｍｏｌ·Ｌ１ＣＯ。系统回收率和加样回收率（Ｘ±ＲＳＤ）分别为１０２．１±２．９％（ｎ＝７）和７９．７±６．８％（ｎ＝１２）；日内、日间精密度（ＲＳＤ）分别为４．４％（ｎ＝１８）和６．１％（ｎ＝１６）。将本法用于４个三卤苯胺和一个三卤苯的体外代谢测定，结果表明，仅２，４，５三氟苯胺在体外能被大鼠肝微粒体、ＮＡＤＰＨ和分子氧代谢生成一氧化碳。苯巴比妥和地塞米松等肝药酶诱导剂能显著提高一氧化碳的代谢转化速率，它们分别为空白对照组的３或８倍。
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| 关 键 词： | 双波长差示一阶导数分光光度法；一氧化碳；定量分析；药物代谢；肝微粒体；三氟苯胺；细胞色素P-450 |
A Dual Wavelength Differential First Derivative Spectro photometric Method for Identification and Determination of Carbon Monoxide Generated During the Microsomal Metabolism of Xenobiotics in vitro |
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| Hong Xiang Xu andZhi Qiang Liu . Jiaxing Institute for Drug Control of Zhejiang Province,Jiaxing , . School of Pharmacy,Zhejiang Medical University,Hangzhou Received November ,, Accepted February . A Dual Wavelength Differential First Derivative Spectro photometric Method for Identification and Determination of Carbon Monoxide Generated During the Microsomal Metabolism of Xenobiotics in vitro[J]. Journal of Chinese Pharmaceutical Sciences, 1997, 0(2) |
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| Authors: | Hong Xiang Xu andZhi Qiang Liu . Jiaxing Institute for Drug Control of Zhejiang Province Jiaxing . School of Pharmacy Zhejiang Medical University Hangzhou Received November Accepted February |
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| Affiliation: | Hong Xiang Xu 1 andZhi Qiang Liu 2 1. Jiaxing Institute for Drug Control of Zhejiang Province,Jiaxing 314001, 2. School of Pharmacy,Zhejiang Medical University,Hangzhou 310006 Received November 28,1995, Accepted February 28,1997 |
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| Abstract: | |
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| Keywords: | Dual wavelength differential first derivative spectrophotometry Carbon monoxide Trifluoroaniline Drug metabolism Hepatic microsomes Cytochrome P 450 |
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