子宫颈鳞癌KI-67、P53、BCL-2蛋白的表达及其在诊断中的应用

目的:探讨KI-67、P53、BCL-2蛋白与子宫颈鳞癌发生的关系及其在诊断中的应用价值。方法:采用免疫组化检测31例子宫颈鳞癌中三种基因蛋白的表达,同时与10例子宫颈上皮内瘤变、13例子宫颈炎作对照。结果:子宫颈鳞癌中K1-67、P53、BCL-2蛋白的表达率分别为85％、45.3％、58％;子宫颈上皮内瘤变中分别为100％、10％、0;子宫颈炎为15％、8％、8％。KI-67蛋白的阳性率子宫颈鳞癌和子宫颈上皮内瘤变均高于子宫颈炎,差异有显著性(P<0.001)。P53和BCL-2蛋白的阳性率子宫颈鳞癌高于子宫颈上皮内瘤变和子宫颈炎,差异有显著性。结论:KI-67、P53、BCL-2均参与了子宫颈鳞癌的发生。KI-67蛋白阳性可能是子宫颈癌变的早期现象,可用于鉴别子宫颈癌前病变和炎性病变。P53和BCL-2蛋白均与子宫颈鳞癌的发生有关,检测此两种蛋白可用于鉴别子宫颈癌和子宫颈上皮内瘤变。采用免疫组化方法联合检测多基因蛋白有助于子宫颈鳞癌的诊断和鉴别诊断。

Expression and application of diagnosis on KI-67, P53 and BCL-2 proteins for squamous cell carcinoma of cervix

Objective To explore the relationship and value in diagnosis among KI-67, P53 and BCL-2 proteins with squamous cell carcinoma of cervix squamous cell carcinoma of cervix. Methods Immunohistochemistry method was used to detect the expression of three types gene proteins in 31 cases of squamous cell carcinoma of cervix, which was compared with 10 cases of cervical intraepithelial neoplasia and 13 cases of chronic cervicitis meanwhile. Results The expression rates of KI - 67, P53 and BCL-2 proteins were 85%, 43. 5% and 58% in squamous cell carcinoma of cervix, were 100% , 10% and 0 in cervical intraepithelial neoplasia, were 15%, 8% and 8% in chronic cervicitis. The positive rates of KI-67 in squamous cell carcinoma of cervix and cervical intraepithelial neoplasia were higher than those in the chronic cervicitis( P <0. 001). The positive rates of P-53 and BCL-2 in squamous cell carcinoma of cervix were higher than those in cervical intraepithelial neoplasia and the chronic cervicitis( P <0. 001). Conclusion KI-67.P53 and BCL -2 are related to the development of squamous cell carcinoma of cervix. The positive of KI-67 may be the early phenomena,and can be used to differentiate from the cervical intraepithelial neoplasia and cervicitis. P53 and BCL-2 are related to the occurrenc of squamous cell carcinoma of cervix,and can be used to differentiate from squamous cell carcinoma of cervix and cervical intraepithelial neoplasia to detect two proteins. It may facilitate the diagnosis and differential diagnosis of squamous cell carcinoma of cervix with immunohisto-chemistry detected polygene proteins at the same time.