环氧合酶-2在喉鳞癌组织中的表达及其临床意义

 【目的】 检测环氧合酶-2（COX-2）在喉鳞癌和喉鳞癌旁非癌组织中表达，分析其与喉鳞癌临床病理因素的关系以及对喉鳞癌患者预后的预测意义。【方法】 收集1997年1月～1998年12月我院收治的81例喉鳞癌患者的临床资料和石蜡块，制作组织芯片，用链霉素亲生物素-过氧化物酶法（SP法）检测81例患者肿瘤组织和癌旁正常喉组织的COX-2表达；采用SPSS10.0软件分析COX-2表达与临床病理因素的关系，采用Cox比例风险模型分析影响预后的因素。【结果】 COX-2在喉鳞癌和癌旁喉组织中的表达率分别为48.15%和0%，（P<0.05）；喉癌COX-2阳性组（5年生存率9.65%）较阴性组（5年生存率97.56%）预后差（P<0.05）；COX-2表达与临床分期、T分期、N 分期、病理分级、发病部位、复发相关（P<0.05）；Cox比例风险模型多因素预后分析结果显示：COX-2表达、临床分期、T分期、病理分级是影响喉癌预后的独立因素（P<0.05）。【结论】 COX-2在喉鳞癌组织中表达上调；COX-2与喉鳞癌患者的病理分化程度、TNM分期、淋巴结转移、复发呈正相关；COX-2表达升高可作为预测喉鳞癌患者预后的独立指标之一。

Expression and Clinical Significance of Cyclooxygenase-2 in Tumor of Laryngeal Squamous Cell Carcinoma

Objective] To detect expression of Cyclooxygenase-2(COX-2 )in laryngeal squamous cell carcinoma (LSCC) and adjacent normal laryngeal mucosa, and to analyze its correlation to clinicopathologic characteristics and prognosis. Methods] Collected information and paraffin-embedded tumor samples of 81 cases LSCC patients, who were treated at Cancer Center of SUN Yat-sen University between 1997 and 1998. Tissue chip was made, and SP immunohistochemistry was performed to analyze COX-2 expression of tumor section and adjacent normal laryngeal mucosa. The SPSS 10.0 software was used to analyze the relationship between the COX-2 expression and the clinicopathologic factors. Cox proportional hazard model was used to analyze the prognosis. Results] The expression rate of COX-2 in laryngeal squamous cell carcinoma and adjacent normal laryngeal tissue were 48.15% and 0%, respectively (P < 0.05). The positive COX-2 expression group (5-OS: 9.65%) had a worse prognosis than negative one (5-OS: 97.56%)(P < 0.05). COX-2 expression was correlated with TNM clinical stage, T stage, N stage, histology grade, subsite of tumor, recurrence (P < 0.05), Cox proportional hazard model multivariate analysis showed: COX-2 expression, clinical stage, T stage, and histology grade were independent prognosis factors of survival of LSCC patients. Conclusions] COX-2 expression is up-regulated in LSCC and positively correlated to tumor cell differentiation, TNM stage, lymph node metastasis and recurrence. COX-2 expression is an independent prognostic factor of LSCC.