A new anti-loxoscelic serum produced against recombinant sphingomyelinase D: results of preclinical trials |
| |
| Authors: | de Almeida Daniel Manzoni Fernandes-Pedrosa Matheus de F de Andrade Rute M Gonçalves Marcelino José Roberto Gondo-Higashi Hisako de Azevedo Inácio de L M Junqueira Ho Paulo Lee van den Berg Carmen Tambourgi Denise V |
| |
| Affiliation: | Laboratório de Imunoquímica, Instituto Butantan, S?o Paulo, Brazil; Programa de Pós-Gradua??o em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Norte, Natal, Brazil. daniel@butantan.gov.br |
| |
| Abstract: | Envenomation by Loxosceles species (brown spider) can lead to local dermonecrosis and to serious systemic effects. The main toxic component in the venom of these spiders is sphingomyelinase D (SMase D) and various isoforms of this toxin are present in Loxosceles venoms. We have produced a new anti-loxoscelic serum by immunizing horses with recombinant SMase D. In the present study, we compared the neutralization efficacy of the new anti-loxoscelic serum and anti-arachnidic serum (the latter serum is used for therapy for loxoscelism in Brazil) against the toxic effects of venoms from spiders of the genus Loxosceles. Neutralization tests showed that anti-SMase D serum has a higher activity against toxic effects of L. intermedia and L. laeta venoms and similar or slightly weaker activity against toxic effects of L. gaucho than that of Arachnidic serum. These results demonstrate that recombinant SMase D can replace venom for anti-venom production and therapy. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
