瘦素、神经肽Y对离体人卵巢颗粒细胞分泌功能的影响

目的　探讨瘦素、神经肽Y(NPY)对离体人卵巢颗粒细胞雌、孕激素生成的影响。　方法　将来自体外受精 胚胎移植的 2 5例离体人卵巢颗粒细胞纯化后 ,在不同浓度瘦素 (1、10、10 0 μg/L)、NPY(1× 10 -8、1× 10 -7、1× 10 -6mol/L)单独或与人绒毛膜促性腺激素 (hCG ,0、2 0IU/L)联合作用下体外培养 ,采用放射免疫方法测定培养液中雌二醇 (E2 )、孕酮(P)的水平。　结果　 (1)无论单独或与hCG联合不同浓度瘦素组E2 、P水平均无显著性差异 (P >0 .0 5 ) ;(2 )NPY浓度 1× 10 -7mol/L时 ,E2 水平显著低于空白对照组 (P <0 .0 5 ) ,但与hCG联合组显著高于单独组 (P <0 .0 5 ) ;(3)NPY浓度 1× 10 -7mol/L、瘦素浓度 10 μg/L组 ,E2 水平明显高于相应浓度NPY单独作用组 (P <0 .0 5 )。　结论　 (1)瘦素不直接影响体外培养颗粒细胞E2 、P的分泌 ,可能通过影响其它因子的作用而间接调节卵巢功能 ;(2 )NPY单独作用时 ,对颗粒细胞E2 的分泌有一定抑制作用 ,但当hCG存在时作用消失 ,说明在正常月经周期中NPY对黄体的生成和维持可能无明显不利影响 ;(3)瘦素可能阻断NPY对颗粒细胞分泌E2 的抑制作用 ,表明二者可能对卵巢功能有相互协调作用。

Effects of leptin and neuropeptide Y on the function of human ovarian granulosa cells in vitro

Objective: To investigate the effects of leptin and neuropeptide Y on the steroidogenesis of human ovarian granulosa cells in vitro.Methods:Human ovarian granulosa cells were isolated from follicular fluid obtained during oocyte retrieval of in vitro fertilization-embryo transfer program and cultured for 2 days with various concentrations of leptin (1,10,100 μg/ml) or neuropeptide Y (1×10 -6 ,1×10 -7 ,1×10 -8 mol/L) alone or both,or the combination with human chorionic gonadotropin (hCG, 0,20 IU/L). The media were collected for estradiol (E_2) and progesterone (P) measurements.Results: (1) Whether hCG existed or not,the adding of leptin did not alter estradiol and progesterone production by human granulosa cell ( P >0.05). (2) Only when the concentration of neuropeptide Y was at 1×10 -7 mol/L,estradiol level was lower than that in the control ( P <0.05). (3) The levels of estradiol in neuropeptide Y (1×10 -7 mol/L ) plus hCG group were significantly higher than those with neuropeptide Y alone ( P <0.05). (4) In the absence of hCG,the levels of estradiol in neuropeptide Y (1×10 -7 mol/L ) plus leptin (10 μg/ml) group were significantly higher than those with neuropeptide Y alone ( P <0.05). Conclusions: (1) Leptin alone produced no direct effect on the secretion of E_2 and P from granulosa cells in vitro;(2) Neuropeptide Y alone may inhibit the secretion of E_2,but when hCG was present,this inhibition was probably blocked;(3) Leptin probably blocked neuropeptide Y’s inhibition on E_2 secretion and there seemed to be some coordination between leptin and neuropeptide Y on the ovarian function.