Allele frequencies of six miniSTR loci in the population of Northern Portugal |
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| Institution: | 1. National Institute of Legal Medicine, I.P., North Delegation, Portugal;2. Health Sciences Faculty, Fernando Pessoa University, Oporto, Portugal;3. Abel Salazar Biomedical Institute, Oporto University, Portugal;1. Division of Medical Virology, Department of Pathology, University of Stellenbosch, P O Box 19063, Tygerberg 7505, South Africa;2. Department of Forensic Pathology, University of Pretoria, Private Bag X20, Hatfield, Pretoria 0028, South Africa;3. Division of Forensic Pathology, Department of Pathology, University of Stellenbosch, P O Box 19063, Tygerberg 7505, South Africa;4. Western Cape Forensic Pathology Service, Cape Town, South Africa;1. Department of Chemistry, Rutgers University, Camden, NJ 08102, USA;2. Center for Computational and Integrative Biology, Rutgers University, Camden, NJ 08102, USA;3. Hamilton Institute, Maynooth University, Ireland;4. Department of Computer Science, Rutgers University, Camden, NJ 08102, USA;5. Department of Plant Biology, Rutgers University, New Brunswick, NJ 08901, USA;1. Division of Medical Virology, Department of Pathology, University of Stellenbosch, P.O. Box 19063, Tygerberg 7505, South Africa;2. Division of Forensic Pathology, Department of Pathology, University of Stellenbosch, P.O. Box 19063, Tygerberg 7505, South Africa;3. Western Cape Forensic Pathology Service, South Africa;1. Department of Neurology, University of Colorado School of Medicine, Aurora, CO, United States;2. Institute of Pathology, Kantonsspital, St. Gallen, Switzerland;3. Department of Pathology, University of Colorado School of Medicine, Aurora, CO, United States;4. Department of Microbiology and Immunology, University of Colorado School of Medicine, Aurora, CO, United States;1. Section of Critical Care Medicine, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA;2. Renal Section, Department of Pediatrics, Baylor College of Medicine, Houston, Texas, USA |
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| Abstract: | A possible approach to try to recover information from degraded DNA is to reduce the size of the PCR products by designing primers that bind as close as possible to the STR repeat region, known as miniSTRs. Allele frequencies and forensic parameters for the six miniSTRs loci D1S1677, D2S441, D4S2364, D10S1248, D14S1434 and D22S1045 were investigated in a sample group consisting of 228 anonymous apparently healthy unrelated individuals living in North of Portugal. The results show that all loci were in Hardy–Weinberg equilibrium. The combined power of discrimination and power of exclusion for the six loci were 0.99999 and 0.9789, respectively. All but one (D4S2364) loci showed a moderate degree of polymorphism (observed heterozygosity >0.6). The allele sizes ranged between 66 and 118 bp in our population, which is beneficial for typing degraded samples than those of a commercial STR kit. |
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