环氧化酶-2 抑制剂联合依托泊苷对肺癌细胞增殖凋亡的影响

 目的 探讨环氧化酶-2（COX-2）抑制剂尼米舒利（NIM）与化疗药依托泊苷（VP-16）联用对肺癌细胞增殖和凋亡的影响。方法 培养人肺癌细胞A549：①NIM（25μmol／L）与VP-16（2～32μg／mL）联合，MTT试验观察干预48h后细胞增殖变化及增殖抑制率；②分为对照组、NIM组（25μmol／L）、VP-16组（8μg／mL）和NIM＋VP．16组，观察12h、24h及48h后A549细胞生长情况，描记生长曲线；流式细胞术检测干预48h后细胞凋亡率。结果 ①VP-16能抑制肺癌A549细胞的增殖，且呈量一效关系（r=-0．908，P〈0．01），NIM与VP-16联用后，抑增殖作用增强，二者有相加或协同作用；②NIM及VP-16均可抑制A549细胞的生长，诱导凋亡，二者联用后作用增强。结论 NIM与VP-16联用可增强对肺癌细胞增殖的抑制和凋亡的诱导，二者具有协同抗肿瘤作用。

The Effects of Nimesulide Combined with Etoposide on Lung Cancer Cell Proliferation and Apoptosis

Objective 　To investigate the effect s of cyclooxygenase-2 selective inhibitor nimesulide (NIM) combined with etoposide (VP-16) on cell proliferation and apoptosis in human lung cancer. Methods 　Human lung cancer A549 cells were incubated and the studies were done as follows : ①NIM (25μmol/ L) combined with VP-16 (2～32μg/ mL) coincubated with A549 cells for 48h , the cell proliferation status and proliferation inhibition rate were detected by MTT reduction assay. ②The cells were divided into control group , NIM (25μmol/ L ) group , VP-16 (8μg/ mL ) group and NIM + VP-16 group . The cells growth activity was observed by growth curve as cells coincubated for 12h , 24h , 48h , and the cell apoptosis rate was analyzed by flow cytomet ry. Results 　①VP-16 could inhibit A549 cells proliferation in a concent ration dependent pattern , which was enhanced as combined with NIM. NIM and VP-16 acted in a synergistic or additive pattern. ②NIM and VP-16 could induce A549 cells apoptosis effectively and the antitumor effects were augmented as they combined. Conclusion 　NIM combined with VP-16 can enhance the effects of growth inhibition and apoptosis induction and they play a synergistic anti-tumor effect in lung cancer cell.