纳米脂质体承载榄香烯诱导C6胶质瘤细胞凋亡的实验研究

目的 探讨纳米脂质体承载榄香烯诱导C6胶质瘤细胞凋亡的作用以及对相关基因caspase-3表达的影响。 方法 以同等剂量的榄香烯－纳米脂质体、普通榄香烯和空白培养液作用于C6胶质瘤细胞，分别于0、48、72 h时通过流式细胞仪检测C6胶质瘤细胞的凋亡情况，以免疫组织化学法检测Caspase-3蛋白的表达。 结果 榄香烯－纳米脂质体和普通榄香烯组细胞瘤于48 h和72 h时出现了明显的凋亡，而且其凋亡率明显高于空白对照组。榄香烯－纳米脂质体和普通榄香烯组细胞瘤48 h和72 h时出现了明显的Caspase-3蛋白表达，而且其蛋白表达率也明显高于空白对照组。 结论 榄香烯具有促进C6胶质瘤细胞凋亡和Caspase-3蛋白表达的作用，经纳米脂质体承载后榄香烯的这种作用有增强的趋势。

Effect of Nano-liposome Sustained Elemene in Inducing Cell Apoptosis of C6 Glioma

OBJECTIVE: To study the effect of nano-liposome sustained elemene in inducing cell apoptosis of C6 glioma and to explore its influence on the expression of caspase-3 gene. METHODS: C6 glioma cells were cultured in medium with the same amount of nano-liposome sustained elemene and common elemene respectively, also in blank medium for control. The status of cell apoptosis was determined by flow cytometry at 0, 48 h and 72 h, and the expression of Caspase-3 protein was measured simultaneously by immunohistochemistry assay. RESULTS: Marked apoptosis presented in cells cultured in the medium with nano-liposome sustained elemene or common elemene at 48 h and 72 h, with the apoptotic rate significantly higher than that in the control. At the same time, Caspase-3 protein expression raised significantly in cells cultured in medium with either kinds of elemene, showing significant difference when compared with that in the control. CONCLUSION: Elemene has significant apoptosis promoting and Caspase-3 protein expression inducing effect on C6 glioma cells, which could be facilitated by nano-liposome bearing.