Evaluation and Development of Automatic Two-Dimensional Measurements of Histomorphometric Parameters Reflecting Trabecular Bone Connectivity: Correlations with Dual-Energy X-Ray Absorptiometry and Quantitative Ultrasound in Human Calcaneum |
| |
Authors: | Nathalie R Portero Monique E Arlot Jean-Paul Roux François Duboeuf Pascale M Chavassieux Pierre J Meunier |
| |
Institution: | (1) INSERM Unité 403, Faculté de Médecine R-Laennec, Université Claude Bernard, Lyon, France |
| |
Abstract: | In osteoporosis, bone fragility results from both bone loss and changes in trabecular microarchitecture, which can be quantified
by bone histomorphometric parameters. Twenty human calcaneum were collected after necropsy. All measurements were performed
at the same anatomical location. Bone histomorphometric parameters were measured on histological slides with an automatic
image analyzer. The aims of our study were (1) to develop automatic measurements of four additional parameters reflecting
trabecular network connectivity and complexity, i.e., trabecular bone pattern factor (TBPf), Euler number/tissue volume (Euler)
according to the three definitions previously reported and to a fourth one established in the laboratory (Eulerstrut.cavity), marrow star volume, and interconnectivity index, and to determine their usefulness in microarchitecture characterization;
and (2) to validate these parameters by evaluating their relationship with dual-energy X-ray absorptiometry and quantitative
ultrasound (QUS) measurements performed on the same samples. The statistical analysis showed that TBPf and Eulerstrut.cavity appeared to be the most significant connectivity parameters, independently of bone quantity (bone mineral density, apparent
density, cancellous bone volume). For QUS, after adjustment for bone quantity, only speed of sound (SOS) was significantly
and negatively correlated to Eulerstrut.cavity. Broadband ultrasound attenuation depends only on bone quantity. In conclusion, TBPf (a strut analysis parameter extrapolable
in three dimensions) and Eulerstrut.cavity (the only bone connectivity parameter reflecting SOS) are two valid bone microarchitecture parameters. These new parameters
were significantly correlated to the established trabecular structure parameters: trabecular thickness or trabecular spacing,
being weakly correlated with SOS. |
| |
Keywords: | Histomorphometry Bone microarchitecture Automatic image analysis Quantitative ultrasound Calcaneum |
本文献已被 PubMed SpringerLink 等数据库收录! |
|