肾癌TIL的分离培养和其表型分析

对两例原发性肾癌患者手术切除肿瘤组织中肿瘤浸润性淋巴细胞（ＴＩＬ）进行了体外分离与培养。结果表明：ＴＩＬ体外扩增倍数分别达３２～２０３倍，对自体肿瘤靶细胞的最高杀伤活性达５３％和６４％，且呈现一定的靶细胞特异性。免疫组化分析结果：未经激活的ＴＩＬ细胞其膜抗原（ＣＤ３，ＣＤ４，ＣＤ８）的表达动态变化不大，但经ＩＬ－２激活的ＴＩＬ细胞随着培养无数的增加，其ＣＤ３细胞数比例及ＣＤ４／ＣＤ８比值上升明显，在培养至３２天时分别达９５％和１．６５。

In vitro isolation and cultivation of tumor-infiltrating Iymphocytes from human renal cell carcinoma and its phenotype analysis

Tumor-infiltrating lymphocytes (TIL) isolated from two renal cell carcinoma were cultured in vitro in the presence of rh IL-2.The results showed that TIL was expanded 32-203 times and exhibited specific strong cytotoxic activity against autologous fresh renal tumor target cells (up to 53% and 640%).Phenotype characterization of the preparations showed that the CD3,CD4 and CD8 phenotype expressions of non-induced TIL cells did not change in the process of cultivation. Whereas,the CD3 expression and the CD/CD8ratio increased significantly to 95% and 1.65 respectively in the culture of activated TIL at 32th day.