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Ultrastructural and immunohistochemical findings in oral hairy leukoplakia
Authors:Xiaolin Zhang  Angelika Langford  Jürgen Becker  Jörg-Peter Rabanus  Hans-Dieter Pohle  Peter Reichart  Hans Gelderblom
Institution:(1) Institute of Stomatology, Beijing Medical University, Haidian Weigongcun, Beijing, P.R. China;(2) Abteilung für Zahnärztliche Chirurgie/Oralchirurgie Nord, Freie Universität Berlin, Föhrer Strasse 15, D-1000 Berlin 65;(3) II. Medizinische Klinik, Universitätsklinikum Rudolf Virchow, Augustenburger Platz 1, D-1000 Berlin 65;(4) Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, D-1000 Berlin 65
Abstract:Summary Three cases of HL from the lateral border of the tongue of male homosexual AIDS patients were investigated by thin section electron microscopy. Keratinocytes contained condensed chromatin in their pyknotic nuclei and a few organelles in the oedematous cytoplasm. Chromatin was in close association to the nuclear membrane and showed a punched-out appearance. Particles typical of the herpes virus group were abundant in the upper two thirds of the epithelium in all three cases. Virus particles were seen frequently in the nuclei of the ballooned keratinocytes, but rarely in cells containing Candida albicans. Viral nucleocapsids were observed budding at the inner nuclear membrane, thereby acquiring the prospective viral envelope. Complete, enveloped virions were found in the endoplasmic reticulum and in the extracellular space. These virions were identified immunohistochemically as Epstein-Barr virus (EBV) using two monoclonal antibodies directed against EBV capsid and membrane antigen, respectively. Candida albicans was observed in the stratum corneum and in the upper layer of the stratum spinosum. Special cytoplasmic tubular structures arranged in parallel bundles were found in koilocytotic cells in addition to characteristic membrane structures composed of undulating convoluted membranes. Epithelial basement membranes were always intact.This study was supported in parts by the Bundesministerium für Forschung und Technologie (grant No. II-022-86) and by an Alexander-von-Humboldt fellowship to Dr. Xiaolin Zhang
Keywords:Hairy leukoplakia  Electron microscopy  Epstein-Barr virus  Candida albicans
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