染料木黄酮通过上调血红素氧合酶-1表达减缓野百合碱诱导的大鼠肺动脉高压

目的研究染料木黄酮对野百合碱诱导的肺动脉高压大鼠肺组织血红素氧合酶-1(heme oxygenase-1,HO-1)表达的影响,探讨其减缓肺动脉高压的可能机制。方法 40只雄性SD大鼠随机分成正常对照组(n=10)、野百合碱组(n=10)、小剂量染料木黄酮治疗组(20μg/kg;n=10)、大剂量染料木黄酮治疗组(80μg/kg;n=10)。监测血流动力学变化及通过电子显微镜观察肺小动脉结构重塑,Westernblot检测肺组织HO-1的表达。结果染料木黄酮治疗组较野百合碱组大鼠平均肺动脉压降低(P<0.01),右心肥厚指数下调(P<0.01),大剂量组更显著(P<0.01)。染料木黄酮治疗组较野百合碱组大鼠肺组织HO-1表达上调(P<0.01),大剂量组更显著(P<0.01)。结论染料木黄酮能减缓野百合碱诱导的大鼠肺动脉高压,可能与上调大鼠肺组织HO-1表达有关。

Genistein attenuates pulmonary arterial hypertension induced by monocrotaline in rats by upregulating the expression of heme oxygenase-1

Objective To study the effect of genistein on the expression of heme oxygenase-1(HO-1) in rats with pulmonary arterial hypertension(PAH) induced by monocrotaline(MCT).Methods Forty male Sprague-Dawley rats were randomly divided into four groups,control group(n=10),model group(n=10),low dose genistein treatment group(20 μg/kg)(n=10) and high dose genistein treatment group(80 μg/kg)(n=10).The hemodynamic parameters were mearsured and the remodeling of pulmonary small arteries was observed by electron microscope(EM).The expression of HO-1 in lung tissues were detected by Westernblot.Results Compared with model group,genistein significantly reduced the elevated mean pulmonary arterial pressure(mPAH) and improved the right ventricular hypertrophy index(RVHI).Meanwhile,the expression of HO-1 in genistein treatment group was higher.Furthermore,these effects of genistein were in a dose dependent manner.Conclusion Genistein attentuates the development of pulmonry arterial hypertension in MCT-treated rats may be induced by upregulation of HO-1 in lung tissues.