| 肾癌G250抗原基因在sp2/0细胞中的表达及其意义 |
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| 引用本文： | 张亚东,吴荣佩,刘雷,李乐军,曹开源,李晓飞.肾癌G250抗原基因在sp2/0细胞中的表达及其意义[J].中山医科大学学报,2008,29(1):16-19. |
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| 作者姓名： | 张亚东 吴荣佩 刘雷 李乐军 曹开源 李晓飞 |
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| 作者单位： | [1]中山大学附属第一医院泌尿外科,广东广州510080 [2]中山大学临床检验标准化中心,广东广州510080 |
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| 基金项目： | 广东省科技攻关项目（2003c31201）;广州市科技攻关项目（200322-E4011） |
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| 摘 要： | 【目的】将G250抗原cDNA片段转染入sp2/0细胞中并获得稳定表达,为G250基因疫苗研究提供实验工具。【方法】用脂质体转染法将鉴定好的重组质粒转染入sp2/0细胞中,经G418筛选得到稳定表达,采用逆转录聚合酶链式反应（RT-PCR）、间接免疫荧光、Western blot检测G250基因在sp2/0细胞中的表达。【结果】转染2周后,筛选出阳性sp2/0细胞克隆,通过RT-PCR证实有G250mRNA转录,间接免疫荧光可以见到阳性细胞,Western blot证实有G250抗原表达。【结论】建立了稳定表达G250抗原的肿瘤细胞模型,为研究基于肾癌G250基因的肿瘤基因疫苗治疗奠定了基础。
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| 关 键 词： | 肾细胞癌（RCC） G250/MN/CAIX（G250）抗原 基因表达 |
| 文章编号： | 1672-3554（2008）01-0016-04 |
| 收稿时间： | 2007-06-01 |
Eukaryotic Expression of Renal Cell Carcinoma G250 Antigen Gene in sp2/0 Cells and Its Significance |
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| ZHANG Ya-dong,WU Rong-pei, LIU Lei, LI Le-jun, CAO Kai-yuan, LI Xiao-fei.Eukaryotic Expression of Renal Cell Carcinoma G250 Antigen Gene in sp2/0 Cells and Its Significance[J].Academic Journal of Sun Yat-sen University of Medical Sciences,2008,29(1):16-19. |
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| Authors: | ZHANG Ya-dong WU Rong-pei LIU Lei LI Le-jun CAO Kai-yuan LI Xiao-fei |
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| Abstract: | Objective] To provide a tool for the therapy of gene vaccine based on G250 gene, we transferred it into sp2/0 cells to obtain stable expression. Methods] The constructed recombinant plasmid was transfected into sp2/0 cells, which were screened by G418, then the positive cells was identified. Results] Two weeks after transfection, we got positive cells. RT-PCR and indirect immunofluorescence methods showed that the G250 gene was expressed in positive sp2/0 cells. Conclusion] The cell model expressing G250 antigen was established, which provided a basis for further exploring the therapy of gene vaccine based on RCC G250 gene. |
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| Keywords: | renal cell carcinoma G250/MN/CAIX antigen gene expression |
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