微囊藻毒素LR抑制小鼠淋巴细胞免疫功能的体外研究

目的：探讨微囊藻毒素LR（microcystin LR,MC-LR）抑制小鼠淋巴细胞免疫功能的可能机制。方法：分离小鼠脾淋巴细胞,分别用含MC-LR为0、1、3、5、10μg/ml的培养液,染毒脾淋巴细胞,MTT法检测MC-LR对小鼠T、B淋巴细胞增殖的影响;ELISA法测定淋巴细胞IL-2的含量;FCM法检测淋巴细胞凋亡率。结果：各MC-LR剂量组淋巴细胞的增殖指数均下降;1μg/mlMC-LR刺激淋巴细胞分泌IL-2,而5、10μg/mlMC-LR组却抑制淋巴细胞分泌IL-2;10μg/mlMC-LR组淋巴细胞凋亡率明显升高（P均〈0.05）。结论：体外培养条件下,MC-LR能明显抑制小鼠脾淋巴细胞增殖,降低淋巴细胞因子IL-2的含量并诱导淋巴细胞凋亡。

Suppression of lymphocyte immune function by MC-LR in vitro

OBJECTIVE: To explore the suppression mechanism of MC-LR on lymphocyte immune function in mice. METHODS: Mice splenic lymphocytes were treated with MC-LR at the dose of 0, 1, 3, 5, 10 μg/ml in vitro. The T and B lymphocyte proliferations were examined by MTT assay. The production of IL-2 in cells was measured by ELISA and the apoptosis rate of lymphocytes was deteceted by FCM. RESULTS: The lymphoproliferation was suppressed by MC-LR at all doses. The production of IL-2 was increased at 1 μg/ml but decreased after treatment with MC-LR at 5 and 10 μg/ml. The rate of lymphocyte apoptosis was increased at 10 μg/ml /n vitro. CONCLUSION: MC-LR could decrease the production of IL-2 of mouse lymphocyte but increase its apoptosis in vitro. MC-LR could markedly inhibit mice lymphocyte proliferation in vitro.