Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis |
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Authors: | Jimenez Joaquin J Jy Wenche Mauro Lucia M Soderland Carl Horstman Lawrence L Ahn Yeon S |
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Institution: | Wallace H Coulter Platelet Laboratory, Division of Hematology/Oncology, University of Miami School of Medicine, 1600 NW 10th Ave, Mail Code R36A, Miami, FL, USA. jjimenez@med.miami.edu |
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Abstract: | BACKGROUND: Endothelial cells (EC) shed endothelial microparticles (EMP) in activation and apoptosis. OBJECTIVES: We compared the antigenic expression of EMP species released during activation as compared to apoptosis, in three cell lines. METHODS: EC from renal and brain microvascular (MiVEC) and coronary macrovascular (MaVEC) origin were incubated with TNF-alpha to induce activation, or deprived of growth factors to induce apoptosis. Antigens expressed on EMP and EC were assayed flow cytometrically and included constitutive markers (CD31, CD51/61, CD105), inducible markers (CD54, CD62E and CD106), and annexin V binding. RESULTS: It was found that in apoptosis, constitutive markers in EMP were markedly increased (CD31>CD105), with a concomitant decrease in expression in EC. Annexin V EC surface binding and annexin V+ EMP were more sharply increased in apoptosis than in activation. In contrast, in activation, inducible markers in EMP were markedly increased in both EMP and EC (CD62E>CD54>CD106). Coronary MaVEC released significantly less EMP than MiVEC. CONCLUSION: EC release qualitatively and quantitatively distinct EMP during activation compared to apoptosis. Analysis of EMP phenotypic signatures may provide clinically useful information on the status of the endothelium. |
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Keywords: | Endothelial microparticles Activation Apoptosis Endothelial cells Phenotype Adhesion molecules |
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