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| 低氧培养的SW480细胞中NDRG1和MMP-2的表达 | |
| 引用本文： | 卢洪,罗国锋,江从庆,钱群,汤华.低氧培养的SW480细胞中NDRG1和MMP-2的表达[J].医学新知杂志,2006,16(5):275-276,279. |
| 作者姓名： | 卢洪 罗国锋 江从庆 钱群 汤华 |
| 作者单位： | 1. 湖北省洪湖市人民医院外科,洪湖,433200 2. 湖北航天医院外科,孝感,432100 3. 武汉大学中南医院大肠肛门外科,武汉,430071 |
| 摘 要： | 目的观察低氧对结肠癌SW480细胞中NDRG1、MMP-2蛋白表达的影响。方法分别在常规(常氧组)和低氧(低氧组)条件下培养结肠腺癌SW480细胞。运用免疫细胞化学染色法检测2组NDRG1蛋白表达,并用图像分析仪测光密度值(OD值);ELISA法检测MMP-2表达。结果低氧组细胞NDRG1呈强阳性表达,而常氧组表达较弱,低氧组细胞NDRG1蛋白表达水平(0．1634-4-0．0079)显著高于常氧组(0．1358±0．0027)(P<0．05);MMP-2表达也显著高于常氧组(P<0．01)。结论低氧诱导了SW480细胞NDRG1、MMP-2表达上调。 |
| 关 键 词： | SW480 缺氧 NDRG1 MMP-2 |
| 收稿时间： | 2006-07-07 |
| 修稿时间： | 2006-07-07 |
Effects of Hypoxia on the Expression of NDRG1 and MMP-2 in Colon Cancer Cell SW480 |
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| Lu Hong Luo Guofeng,Jiang Congqing,et al.Effects of Hypoxia on the Expression of NDRG1 and MMP-2 in Colon Cancer Cell SW480[J].Journal of New Medicine,2006,16(5):275-276,279. | |
| Authors: | Lu Hong Luo Guofeng Jiang Congqing |
| Institution: | Lu Hong Luo Guofeng,Jiang Congqing,et al 1 Department of Surgery,Renmin Hosipital of Honghu,Honghu 433200 2 Department of Surgery,Hangtian Hosipital of Hubei,Xiaogan 432100 3 Department of Colorectal Surgery,Zhongnan Hosipital of Wuhan University,Wuhan 430071 |
| Abstract: | Objective To observe effects of hypoxia on the expression of NDRG1 and MMP-2 in colon cancer cell SW480.Methods NDRG1 protein was measured by immunocytochemistry, and MMP-2 protein was measured by ELISA. Results The level of NDRG1 proteins expression in SW480 cells was significantly higher in the hypoxia group than in the normoxia group (P<0.05).The results of ELISA revealed that the level of MMP-2 protein expression in culture medium was significantly higher in the hypoxia group than in the normoxia group (P<0.05). Conclusion Hypoxia induces the expression of NDRG1 and MMP-2. |
| Keywords: | cell line SW480 hypoxia NDRG1 MMP-2 |
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