| 利血平对多巴胺所致PC12细胞程序性死亡的影响 |
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| 引用本文： | 方刚,凌立君,侯亚义,韩晓冬.利血平对多巴胺所致PC12细胞程序性死亡的影响[J].医学研究生学报,2002,15(2):109-111. |
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| 作者姓名： | 方刚 凌立君 侯亚义 韩晓冬 |
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| 作者单位： | 南京大学医学院免疫微生物学教研室,江苏南京,210093 |
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| 摘 要： | 目的 :探讨帕金森病黑质多巴胺神经元的死亡机制。 方法 :用MTT法及流式细胞仪检测和观察囊泡单胺类转运体 (VMAT)抑制剂利血平对多巴胺所致的毒性作用和细胞程序性死亡的影响。 结果 :利血平 ( 10 0 0nmol/L)作用于大鼠嗜铬细胞瘤 (PC12 )细胞 96h ,对细胞无毒性效应 ;多巴胺 ( 0 .4mmol/L)作用于PC12细胞 4 8h即出现毒性效应 ;利血平 ( 10 0 0nmol/L)与多巴胺能够明显增强多巴胺 ( 0 .4mmol/L)的毒性效应 (P <0 .0 1) ,二者的协同毒性效应还具有时间依赖性。当多巴胺浓度 ( 0 .2mmol/L)较低时 ,协同毒性效应在药物作用 96h才显现出来。同时毒性效应主要是通过诱导细胞程序性死亡来实现的。 结论 :VMAT功能下降加重或触发多巴胺的内源性毒性 ,诱导多巴胺神经元的程序性死亡 ,可能参与帕金森病的发病机制。
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| 关 键 词： | 帕金森病 利血平 多巴胺 PC12细胞 |
| 文章编号： | 1008-8199(2002)02-0109-03 |
| 修稿时间： | 2001年9月26日 |
The effect of reserpine on dopamine-induced apoptosis in PC12 cells |
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| FANG Gang,LING Li jun,HOU Ya yi,HAN Xiao dong.The effect of reserpine on dopamine-induced apoptosis in PC12 cells[J].Bulletin of Medical Postgraduate,2002,15(2):109-111. |
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| Authors: | FANG Gang LING Li jun HOU Ya yi HAN Xiao dong |
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| Abstract: | Objectives: To study the mechanism of dopaminergic neuronal death in substantia nigra of Parkinson disease. Methods:The effects of reserpine on dopamine induced cytotoxicity and apoptosis in PC12 cells were detected by MTT assay and flow cytometry. Results: Reserpine (1 000 nmol/L) itself had no direct poisonous effect on PC12 cells, but it increased dopamine induced cytotoxicity and apoptosis in PC12 cells. These effects were dopamine concentration related, and were also dependent on the drug exposure time. Conclusions: Inhibition of VMAT induced apoptosis in PC12 cells, which may involved in the dopaminergic neuronal death of Parkinson disease. |
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| Keywords: | Parkinson disease Reserpine Dopamine PC12 cells |
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