延藿骨宝对实验性大鼠骨质疏松的骨密度和生物力学影响

目的:探讨延藿骨宝对类固醇性大鼠骨骼的骨密度和生物力学影响。方法:采用3月龄雄性SD大鼠28只,随机分为基础对照组、年龄对照组、激素模型组和延藿骨宝治疗组。3个月后取股骨和第5腰椎行骨密度测定和力学性能检测。结果:与对照组比较,激素模型组股骨和腰椎的骨密度均出现下降,除股骨干三点弯曲试验所承受的载荷明显减少17.1％(P<0.05)外,其余的力学参数都有减少的趋势;应用延藿骨宝治疗后能防止类固醇所致的骨密度和力学性能下降。结论:长期使用类固醇会使大鼠股骨和腰椎的骨密度和力学性能下降,应用延藿骨宝能扭转类固醇性大鼠骨骼的骨密度和力学性能下降。

Bone Mineral Density and Biomechanical Effects of Chinese Herbal Drug Yanhuogubao on Experimental Osteoporosis in Rats

Objective: To explore bone mineral density and biomechanical effects of Chinese herbal drug Yanhuogubao on steroid hormone-induced osteoporosis in rats. Methods: Male, 3-month-old Sprague-Dawley rats were randomly divided into 4 groups(n = 7), the basal group, the age control group, the hormone group (4.5 mg/kg prednisone acetate, twice a week) and Yanhuogubao (1.5 g/kg/d) group. The bone mineral densities and the mechanical characters measurements of femurs and 5th lumbar vertebrae in every group were made by using DEXA and SWD-10 electronic universal material testing machine. Results: The bone mineral densities on femora and 5th lumbar vertebrae in hormone group decreased, and the loads imposed on the femoral trunk in three-point bending test decreased by 17.1%(P< 0.05), though the rest parameters tended to decrease in comparison with control group. In the meantime, Yanhuogubao can prevent prednisone acetate-induced the bone mineral densities and mechanical characters of femurs and lumbar vertebrae in rats from decline, compared with hormone group. Conclusion: When steroid hormone was used for a long time, the bone mineral densities and mechanical characters of femora and 5th lumbar vertebrae in the rats would be led to decrease, on the contrary, Yanhuogubao could arrest the decline of bone mineral densities and mechanical characters of rat skeleton.