中医"肺应秋"与SD大鼠免疫功能相关性的实验研究

目的通过对春秋季节SD大鼠免疫功能的测定,探讨中医“肺应秋”的免疫学机制内涵,为呼吸系统季节性发病的病理生理机制的认识提供实验依据。方法采用松果腺摘除大鼠模型,测定春分、秋分大鼠脾脏指数和胸腺指数、肺泡巨噬细胞吞噬功能。结果正常组脾脏指数和胸腺指数,秋分明显低于春分,有非常显著差异(P<0.01);伪手术组秋分低于春分,有显著差异(P<0.05);手术组春分和秋分时差异不明显。正常组肺泡巨噬细胞吞噬功能秋分明显低于春分,有非常显著差异(P<0.01);伪手术组与手术组均使春分和秋分差异消失。结论SD大鼠脾脏指数、胸腺指数、肺泡巨噬细胞吞噬功能存在秋低春高的季节节律,这可能是秋季季节性呼吸系统多发病的病理生理学基础之一。SD大鼠松果腺-褪黑素是免疫功能季节性节律变化的中介物质基础,其调节机制具有复杂性、选择性和间接性。中医“肺应秋”具有免疫学物质基础,与松果腺的中介调节作用密切相关。

Experimental Research on the Relationship between TCM Lung Corresponding with the Autumn and Immune Functions of SD Rats

Objective Through testing the immune functions of SD rats in spring and autumn, the internal mechanisms that lung corresponding with the autumn of TCM were explored, in order to provide the experimental evidence for the pathological and physiological mechanisms of respiratory system seasonal diseases. Method The pineal extirpating rat model was employed, and the indexes of spleen and thymus gland and the phagocytosis function of alveolar macrophage were observed at vernal equinox and autumnal equinox respectively. Result The indexes of spleen and thymus in the normal group and the false operation group at autumnal equinox were significantly lower than those at vernal equinox (P<0.01 or P<0.05). While in the operation group, there was no significant difference in the indexes of spleen and thymus between vernal equinox and autumnal equinox. In the normal group, the phagocytosis function of alveolar macrophage at autumnal equinox was significantly lower than that at vernal equinox (P<0.01). While in the false operation group and the operation group, there were no significant differences in the phagocytosis function between the two seasons. Conclusion There are seasonal rhythms of the functions in the spleen, thymus and alveolar macrophage in SD rats, which are lower in autumn than that in spring. The melatonin of the pineal gland of SD rats maybe is the medial material relating with the seasonal changes of immune function. The lung corresponding with the autumn of TCM is of immunological material base, which maybe has close relationship with medial modulating of pineal gland.