单克隆抗体在寄生虫病免疫诊断中的应用

单克隆抗体以其特异性高、效价高、易纯化等优点得到了广泛的应用,该文对单克隆抗体应用于乳胶凝集试验、间接血凝试验、免疫荧光检测、流式细胞仪检测、免疫组织化学、放射免疫测定法、免疫印迹技术、免疫酶染色技术和斑点免疫金银染色法、酶联免疫吸附试验、金免疫渗滤法、免疫层析技术和免疫色谱技术等12种免疫学诊断方法,及其在寄生虫病诊断中的应用作一综述.

Applications of monoclonal antibodies in the immunodiagnosis of parasitic diseases

The monoclonal antibody has been widely applied owing to its advantages of high specificity,efficacy and easy purification.The application of twelve immnodiagnosis methods including latex agglutination test(LAT),indirect haemagglutination test(IHA),immunofluorescent assay(IFA), flow cytometry test (FCM),immunohistochemistry test,radioimmunoassay(RIA),immunoblot test,immunoenzyme and immunogold-silver staining test,enzyme linked immmunosorbent assay(ELISA),immunogold filtration,immunochromatography and immunochromatographic test(ICT)with monoclonal antibody in the immunodiagnosis of parasitic diseases was reviewed in this paper.