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免疫层析法用于表面抗原检测
引用本文:李晨旭,刘洪阳,孙长杰,刘成. 免疫层析法用于表面抗原检测[J]. 吉林大学学报(医学版), 1998, 0(4)
作者姓名:李晨旭  刘洪阳  孙长杰  刘成
作者单位:长春结核病院检验科
摘    要:目的:建立一种更方便、快速、安全的检验血清HBsAg的手段。方法:采用胶体金免疫层析分析方法。结果:检测临床及血库血清样品189例,与酶联免疫吸附试验(ELISA)对照比较,二者检测符合率为98.9%,检测灵敏度可达5μg/L以下。结论:该方法检测灵敏度、特异性等指标已接近ELISA水平,并且检测操作更方便、快速,有希望发展成为一种新的临床检测方法。

关 键 词:免疫层析  胶体金  表面抗原  试纸

Diagnosis of HBsAg by immunochromatography
Li Chenxu,Liu Hongyang,Liu Cheng,Sun Changjie. Diagnosis of HBsAg by immunochromatography[J]. Journal of Jilin University: Med Ed, 1998, 0(4)
Authors:Li Chenxu  Liu Hongyang  Liu Cheng  Sun Changjie
Abstract:Objective:To develop a methed to detect serum HBsAg which was more simple.rapid and safe.Metheds:Colloidal gold immunochromatography was selected selected. Results: In detecting 189 serum sapmles fromclinic and blood bank, this method had a coincidence rate of 98. 9% with ELISA. Detection sensitivity couldreach 5μg/L or even lower. Conclusion: The detection sensitivity and sepecificity by this method approach thoseby ELISA and it could be developed to be a new clinical detecting method because of its simpleness and rapidness in operation.
Keywords:immunochrormatography  cllidal gold  HBsAg  strip
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