川芎嗪对辐射致血虚证小鼠骨髓细胞蛋白质表达的影响

目的:观察川芎嗪对辐射致血虚证小鼠骨髓细胞蛋白质表达的影响,探讨川芎嗪补血作用的分子机制。方法:采用3.5 Gy60Coγ射线全身1次性照射,制备小鼠血虚证模型;骨髓集落细胞培养,观察并计数粒系-巨噬细胞集落生成单位(CFU-GM)、爆增型红细胞集落生成单位(BFU-E)、红细胞集落生成单位(CFU-E)、混合集落生成单位(CFU-mix)集落数;利用蛋白质组学寻找差异表达蛋白质。结果:辐射后小鼠骨髓CFU-GM、BFU-E、CFU-E、CFU-mix集落数明显减少,川芎嗪能使其显著回升并部分逆转辐射后蛋白质表达的变化,使小鼠骨髓细胞5种蛋白质表达回升,5种回落。结论:川芎嗪可促进辐射致血虚证小鼠骨髓造血祖细胞增殖,调节多种骨髓细胞蛋白质的表达,后者可能是川芎嗪促进造血细胞生长和增殖的重要机制之一。

Effect of ligustrazine on protein expression of bone marrow cell of blood deficiency mice induced by radiation

Objective:To observe the effect of ligustrazine on protein expression of bone marrow cell of blood deficiency mice induced by radiation and to study the molecular mechanism of ligustrazine in blood enriching function.Methods:The blood deficiency mice were induced by using a single dose of 3.5 Gy ~(60)Co γ radiation.Myeloid colony cell was cultured and the number of CFU-GM,BFU-E,CFUE and CFU-mix were observed and counted.By using proteomics methods,such as two-dimensional electrophoresis(2-DE),image analysis,in-gel digestion,and mass spectrometry with bio-informatics,the differential expression proteins were separated,analyzed and identified.Results: After irradiation,colony number of CFU-GM,BFU-E,CFU-E and CFU-mix decreased significantly.Ligustrazine can raise significantly the colony number of CFU-GM,BFU-E,CFU-E and CFU-mix of mice bone marrow and reverse partly the change of protein expression induced by irradiation,which makes five kinds of down-regulated proteins raise again and five kinds of up-regulated proteins fall back.Conclusion: Ligustrazine can promote hemopoietic progenitor cell proliferation of bone marrow of blood deficiency mice induced by radiation and regulate the expression of many kinds of bone marrow cell proteins.The latter effect could be one of the main mechanisms that ligustrazine can promote hemopoietic cell growth and proliferation.