86例成人急性髓性白血病免疫表型分析

常规使用10种单抗，用流式细胞仪（FACS）或荧光显微镜（FM）间接免疫荧光标记法检测了86树成人急性髓性白血病（AML）患者骨髓细胞的免疫标志。结果显示，髓系标志中CD33、CD13诊断价值较高，而CD15、CD14阳性率低。31．4％AML有淋系抗原表达，无单抗标记AML占16．3％，47．6％的患者表达CD34.FACS检测较FM更敏感、特异。

Immunophenotypic Analysis of 86 Patients With Acute Myeloid Leukemia in Adults

Immunophenotypic analysis was performed in 86 adults patients with acute myeloid leukemia (AML) by means of indirect immunonuorescence and now cytometry (FACS,n=18) or fluorescence microscopy (FM,n = 68). 10 murine monoclonal antibodies were used directe at myeloid (CD13,CD33,CD15 or CD14),lymphoid (CD2,CD3,CD7,CD10,CD19,CD22),or stem cell (CD34) antigens. The results showed that CD13 and CD33 were the most useful positive diagnostic marks as compared with CD15 and CD14 which were less commonly positive. 31. 4% of cases had lymphoid antigens. 16. 3% of cases had no response to all the above 10 antibodies. CD34 was detected to be 47. 6%. FACS was more sensitive and specific than FM in immunophenotypic analysis.