Translocation (11;14)(q13;q32) and preferential involvement of chromosomes 1, 2, 9, 13, and 17 in mantle cell lymphoma. |
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| Authors: | B Espinet F Solé S Woessner F Bosch L Florensa E Campo D Costa E Lloveras R M Vilà C Besses E Montserrat J Sans-Sabrafen |
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| Institution: | Laboratori de Citologia Hematològica, Laboratori de Referència de Catalunya, Hospital de l'Esperan?a, Barcelona, Spain. |
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| Abstract: | We have studied 13 cases of histologically confirmed mantle cell lymphomas (MCL) combining cytological-immunological features with conventional cytogenetics and in situ hybridization (ISH) techniques. Peripheral blood smears and lymph node biopsies expressed the typical mantle zone pattern with alpha B-cell phenotype. Most of the cases (11 of 13) had lymphomatous cells in the peripheral blood. Chromosome analysis was carried out on lymphoid cells from peripheral blood and/or lymph node biopsies. Phytohemagglutinin (PHA) and phorbol 12-myristate 13 acetate (TPA) were used as mitogens. Biotin-labeled libraries of whole chromosomes implicated in complex karyotypes were used to improve their interpretation. Clonal chromosome abnormalities were found in 10 of 13 patients (77%); 7 of these had a complex abnormality. The most frequent recurrent structural abnormalities were: t(11;14)(q13;q32), involvement of chromosome 1 (der1], del1], dup1]), chromosome 2 (del2], der2]), chromosome 9 (der9], -9), chromosome 13 (add13], t13q]), and chromosome 17 (add17], der17], t17q]). The most frequent numerical abnormalities were monosomy 21 and loss of the Y chromosome. |
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