Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family |
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| Authors: | Luo Cong Zhu Yongliang Jiang Tiejun Lu Xiaoyong Zhang Wo Jing Qifeng Li Jia Pang Linrong Chen Kangjie Qiu Fuming Yu Xiuyan Yang Jiahui Huang Jian |
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| Affiliation: | Cancer Institute, Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou 310009, China. |
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| Abstract: | Matrine, one of the main active components from the dry roots of Sophora flavescence, was known to induce apoptosis in a variety of tumor cells in vitro. However, the molecular mechanism of cell apoptosis induced by Matrine remains elusive. Here, we investigated the apoptosis in Matrine-treated human gastric cancer MKN45 cells. The results showed that Matrine could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Further immunoblots revealed that in Matrine-treated cells, caspase-3, -7 were activated and the pro-apoptotic molecules Bok, Bak, Bax, Puma, and Bim were also up-regulated. Our results suggested that Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family. |
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| Keywords: | Bcl-2, B cell lymphoma-2 Bad, Bcl-2 antagonist of cell death Mcl-1, myeloid cell leukaemia sequence 1 BH3, Bcl-2-homology domain-3 Bak, Bcl-2 antagonist/killer Bax, Bcl-2-associated X protein Bok, Bcl-2-related ovarian killer Bid, BH3-interacting domain Bim, Bcl-2-interacting mediator of cell death Puma, p53-up-regulated modulator of apoptosis DMSO, dimethyl sulfoxide MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide |
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