碳化二亚胺交联的胶原-硫酸软骨素支架材料构建人工真皮的研究

目的 探讨以胶原—硫酸软骨素支架材料构建人工真皮的可行性。方法 以碳化二亚胺(EDC)为交联剂构建胶原—硫酸软骨素(CS)支架体系。通过光电子能谱、扫描电镜、HE染色观察及力学性能测试等对材料的理化性能进行表征。同时分离培养人胚真皮成纤维细胞，将体外扩增的成纤维细胞接种在支架上，制备人工真皮。通过组织学、免疫组织化学，与传统的胶原海绵人工真皮的性质进行比较。结果 胶原—CS支架是三维多孔海绵状结构且空隙均匀，与胶原支架比较，断裂强度提高，并且材料表面极性基团增加。成纤维细胞在胶原—CS支架材料上生长良好，形成人工真皮。结论 胶原—CS人工真皮具有优越的生物学和力学性能，成纤维细胞在胶原—CS支架上黏附、增殖情况优于胶原真皮。

CONSTRUCTION OF ARTIFICIAL DERMIS ON COLLAGEN-CHONDRONTIN SULFATE SCAFFOLD CROSSLINKED BY 1-ETHYL-3-(13-DIMETHYL AMINOPROPYL) CARBODIIMIDE

Objective To construct artificial dermis on collagen chondrontin sulfate(CS) scaffold. Methods Collagen was compounded from CS and 1 ethyl 3 (13 dimethyl aminopropyl) carbodiimide(EDC) used as a crosslinker. Physical and chemical properties of the scaffold were characterized by electron spectroscopy for chemical analysis(ESCA), scanning electron micrograph(SEM), HE staining, and mechanical property test. Dermis fibroblasts were isolated from human embryo and were cultured on the scaffolds. Through histological testing, immunohistochemical testing and biochemical property testing, the property of collagen CS artificial dermis was compared with that of collagen spongy artificial dermis. Results Collagen CS had three dimension structure with porous. Compared with collagen scaffold, the mechanical property of collagen CS scaffold improved. There were more polar groups on the surface of collagen CS scaffold. The fibroblasts on the collagen CS scaffold grew well, and artificial dermis was constructed. Conclusion Collagen CS artificial dermis has more excellent biological and mechanical properties. Fibroblasts attach and proliferate better on collagen CS scaffold than on collagen scaffolds.