流式荧光杂交法检测人乳头瘤病毒的临床应用

目的探讨流式荧光杂交法检测人乳头瘤病毒(HPV)的临床应用价值。方法采集苏州地区1 406例妇科就诊患者宫颈脱落细胞,用流式荧光杂交技术检测HPV基因型,并对HPV感染率、基因亚型和年龄分布等进行分析。结果检出HPV感染576例(40.97%),其中单一亚型感染392例(27.88%),多重亚型感染184例(13.09%);采用流式荧光杂交法可同时检测26种HPV基因亚型,最常见的亚型为HPV-6、11、16、52、58等;单纯HPV低危亚型感染117例,占阳性病例的20.31%,高危亚型感染365例(63.37%);50～59岁年龄组HPV感染率最高(52.24%),30～39岁年龄组感染率最低(34.02%);并且,随年龄增加低危亚型感染率逐步降低,高危亚型感染率却呈明显上升趋势。结论本地区妇科患者中HPV感染率较高,流式荧光杂交法是检测HPV感染的有效方法,开展HPV分型检测对宫颈病变的防治意义重大。

Clinical application of flowcytometry fluorescence hybridization method in human papillomavirus genotype detection

Objective To discuss the clinical application of flowcytometry fluorescence hybridization method in human papillomavirus genotype detection. Methods The cervical cast off cells of 1 406 patients in Suzhou area were collected,and the HPV genotypes of the samples were analyzed with flowcytometry fluorescence hybridization meth- od. The HPV infection rate, infectious status of various genotypes and age distribution of HPV were analyzed as well. Results 576 cases were positive（40.97%） ,in which single genotype infection rate was 27.88%（392/576） ,and multiple genotypes infection rate was 13.09% （184/576）. The 26 genotypes of HPV could be tested simultaneously with flowcytometry fluorescence hybridization method, and among them the common subtypes were HPV-6,11,16, 52,58, etc. Among all the HPV-infected patients, 117 cases （20.31%） were just low risk genotype infection, while high risk HPV infection was in 365 cases（63.37%）. The highest HPV infection rate was found at the age group of 50-59 years（52.24%） and the lowest HPV prevalence was found at the age group of 30-39 years（34. 02%）. Along with the increasing age,the infection rate of high risk HPV was increased gradually and the low risk HPV was de- creased. Conclusion The positive rate of HPV infection in local area is rather high. flowcytometry fluorescence hy- bridization method is the most effective method to detect the HPV infection,and it can be of great value to the pre- vention and treatment of cervical diseases.