痰标本铜绿假单胞菌224株5年耐药变迁

目的分析解放军第一一八医院2006～2010年痰标本铜绿假单胞菌检出情况及耐药谱变化,为指导临床控制肺部铜绿假单胞菌感染及合理使用抗菌药物提供参考。方法用自编Excel软件从LIS数据库中采集2006年1月至2010年12月该院住院患者送检痰标本中分离的铜绿假单胞菌共224株。菌株鉴定和药敏试验采用法国生物梅里埃公司的ATB Expression细菌鉴定系统和药敏板条,并用Excel透视图表进行铜绿假单胞菌检出率及耐药分析,率的差异性比较用u检验。结果 5年来该院住院患者送检痰标本778份,共分离检出致病菌571株,其中铜绿假单胞菌224株,占总检出菌的39.2%。亚胺培南耐药株166例,占铜绿假单胞菌的74.1%。按亚胺培南敏感程度分组后,其对常用抗菌药物的耐药率差异有统计学意义(P<0.01)。结论痰标本分离的铜绿假单胞菌对碳青霉烯类抗菌药物耐药率高,耐亚胺培南铜绿假单胞菌对常用抗菌药物的耐药率亦明显高于非耐药株。

5-year drug resistance changes of 224 strains of Pseudomonas aeruginosa isolated from sputum specimens

Objective To analyze the detection situation of Pseudomonas aeruginosa from sputum specimens and its drug resistance change in this hospital during 2006-2010 to provide the reference for controlling pulmonary infection of Pseudomonas aeruginosa and reasonably using antibiotics. Methods 224 strains of Pseudomonas aerugi- nosa isolated from the inpatientsp sputum specimens between January 2006 and December 2010 were collected from the databank of laboratory information system（MS） by the self-made Excel software. The strain identification and the antimicrobial susceptibility test were performed using ATB Expression system and the drug sensitivity strip. The de- tection rate of Pseudomonas aeruginosa and the drug resistance analysis were performed using the Excel perspective chart,and the differences significance of rates was conducted with u-test. Results 779 inpatient sputum specimens were detected during 5 years and 571 strains of pathogenic bacteria were isolated,including 224 strains of Pseudo- monas aeruginosa,accounting for 39.2% of total detected bacteria. 166 strains were resistant to imipenem,occupying 74.1% of Pseudomonas aeruginosa. After grouping according to the imipenem sensitive degree,its resistance rates to commonly used antimicrobials had statistical difference （P 〈0. 01）. Conclusion Pseudomonas aeruginosa isolated from sputum specimens has the high resistance rate to carbapenems drugs（74.1 %）. The resistance rate to commonly used antibacterials in imipenem-resistant Pseudomonas aeruginosa is also higher than that in non-resistance strains.