慢性乙肝患者HBeAg和HBV-DNA载量与ALT水平相关性探讨

目的探讨慢性乙肝患者血清中乙型肝炎e抗原(HBeAg)和乙型肝炎病毒DNA(HBV-DNA)载量与丙氨酸氨基转移酶(ALT)水平之间的关系,为临床诊治提供参考。方法收集380例慢性乙肝患者标本采用ELISA法进行HBeAg检测,血清HBV-DNA定量采用实时荧光定量PCR法,ALT采用全自动生化法。结果Ⅰ组与Ⅱ组HBeAg阳性率差异无统计学意义(P=0.052)。Ⅰ、Ⅱ组分别与Ⅲ组比较,HBeAg阳性率差异均有统计学意义(P<0.01)。HBV-DNA载量与ALT水平存在一定的关联(P=0.000),等级相关系数为0.398。结论慢性乙肝患者不能单靠某项检验指标来判断是否进行抗病毒治疗,在决定是否需抗病毒治疗时,需联合、动态检测这些指标。

Correlation research between serum HBeAg and HBV-DNA with ALT level in patients with chronic type B hepatitis

Objective To explore the relationship between serum HBeAg and HBV-DNA with the AI.T level in order to provide reference for clinical diagnosis. Methods 380 cases of chronic type B hepatitis were collected. Set um HBeAg was detected by using ELISA method,HBV-DNA was quantitated by the real time discrimination assay and fluorescence quantitative PCR assay and ALT was detected by the fully automatic biochemistry assay. Results There was no statistical difference between the group Ⅰ andⅡ （P=0. 052）. Comparing the group Ⅲ with the group Ⅰ and Ⅱ respectively,there was statistical difference in the HBeAg positive rate（P〈0.01）. The HBV-DNA and the ALT level had certain relationship（P=0. 000） ,the Spearman correlation parameter was 0. 398. Conclusion The pa tients with chronic type B hepatitis cannot be estimated by only one test index before antiviral treatment, these test indexes need to be jointly and dynamically detected in order to determine whether adopting antiviral therapy.