| 实时荧光PCR技术在手足口病检查中的应用 |
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| 引用本文： | 龙健灵,叶俊琴,李文瑞,彭琪,何月敬,程庆秋. 实时荧光PCR技术在手足口病检查中的应用[J]. 检验医学与临床, 2013, 10(5): 522-523 |
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| 作者姓名： | 龙健灵 叶俊琴 李文瑞 彭琪 何月敬 程庆秋 |
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| 作者单位： | 广东省东莞市石龙博爱医院儿科研究所 5233251 |
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| 摘 要： | 目的采用实时荧光聚合酶链反应(PCR)法检测手足口病患儿病原体。方法收集433例疑似手足口病患儿的粪便标本进行肠道病毒通用型、肠道病毒71型(EV-71)和柯萨奇病毒16型(CA-16)鉴别与分析。结果实时荧光PCR法检测433例患儿肛拭子标本使用通用型试剂盒检出366例阳性,其中66例为EV-71感染,151例为CA-16感染。结论 EV-71和CA-16肠道病毒是手足口病的主要病原体,该地区2011年以CA-16感染为主,实时荧光PCR技术对手足口病的病原诊断具有重要价值。
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| 关 键 词： | 手足口病 肠道病毒 实时荧光聚合酶链反应(PCR) |
Application of real-time fluorescence PCR in detection of HFM D |
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| LONG Jian-ling , YE Jun-qin , LI Wen-rui , PENG Qi , HE Yue-jing , CHENG Qing-qiu. Application of real-time fluorescence PCR in detection of HFM D[J]. Laboratory Medicine and Clinic, 2013, 10(5): 522-523 |
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| Authors: | LONG Jian-ling YE Jun-qin LI Wen-rui PENG Qi HE Yue-jing CHENG Qing-qiu |
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| Affiliation: | (Pediatric Research Institute, Shilong Boai Hospital, Dongguan, Guangdong 523325, China) |
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| Abstract: | Objective To detect the pathogens of hand,foot and mouth disease（HFMD） by real-time fluores cence PCR. Methods The fecal samples in 433 children patients with suspected HFMD were collected for the identi- fication and analysis of universal intestinal virus,EV-71 and CoxA-16. Results Among 433 rectal swab specimens de- tected by real-time fluorescence PCR, 366 specimens were positive,in which, 151 specimens were CA-16 infection and 66 specimens were EV-71 infection. Conclusion CoxA-16 and EV 71 viruses are the main pathogens in HFMD. Cox A-16 virus is the main pathogen of HFMD in the native area this year. Real-time fluorescence PCR technique has im- portant value in the pathogen diagnosis of HFMD. |
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| Keywords: | HFMD intestinal virus real-time fluorescence PCR |
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