去白细胞滤器滤除白细胞失败原因分析

目的研究去白细胞滤器滤除白细胞时导致滤盘不通的血液因素。方法选择40份去白细胞滤器滤白失败的血液标本和50份正常的血液标本,分别检验其血液流变学指标(全血黏度,红细胞刚性指数,红细胞聚集指数),血细胞比容,纤维蛋白原浓度,三酰甘油浓度。若疑有冷凝集素的血样做血清冷凝集素效价试验。结果全血黏度(低切、高切)和红细胞刚性指数增高(P<0.05),以及冷凝集素水平增高对白细胞滤器滤除白细胞有影响。血细胞比容、纤维蛋白浓度、三酰甘油浓度的高低不会影响白细胞滤除(P>0.05)。结论全血黏度、红细胞刚性指数、冷凝集素是导致去白细胞滤器滤除白细胞失败的主要血液因素。

Analysis on failure causes of leukocyte filtration by leukocyte depletion filter

Objective To research the blood factors causing the filter plate blocking during the leukocyte fil- tration by leukocyte depletion filter. Methods 40 blood samples of leukocyte filtration failure and 50 normal blood samples were selected and performed the detection of the hemorheological indexes（whole blood viscosity,erythrocyte rigidity index, erythrocyte aggregation index）, hematocrit, fibrinogen concentration and triglyceride concentrations. In suspected the cold agglutinin blood samples, serum cold agglutinin titer test was performed. Results The whole blood viscosity（low cut,high cut） and erythrocyte rigidity index were increased（P〈0.05）. The cold agglutinin level affected the leukocyte filtration by leukocyte depletion filter. The hematocrit, fibrinogen concentration and triglyceride concentrations had no influence on the leukocyte depletion（P〉0.05）. Conclusion Whole blood viscosity,erythrocyte rigidity index and cold agglutinin are the major blood factors leading to the failure of leukocyte filtration by the leuko- cvtes leukocvte depletion filter.