毛细支气管炎和急性上呼吸道感染患儿血清可溶性TRAIL及其受体TRAILR1、TRAILR4的表达

【目的】 观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其受体(sTRAILR1和sTRAILR4)在毛细支气管炎和急性上呼吸道感染患儿中的表达,探讨它们在呼吸道感染炎症机制中的作用。 【方法】 ELISA 法检测毛细支气管炎和急性上呼吸道感染患儿及健康对照组儿童血清sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度。 【结果】 毛细支气管炎组和急性上呼吸道感染组患者血清中sTRAIL、sTRAILR1和sTRAILR4的蛋白浓度分别为(85±38)、(71±19)、(167±97);(78±28)、(61±15)、(139±72) pg/mL均显著高于对照组(55±19)、(45±14)、(56±38) pg/mL(P均<0.01);毛细支气管炎组sTRAILR1的蛋白浓度显著高于急性上呼吸道感染组(P<0.05),两组sTRAIL和sTRAILR4的蛋白浓度相比差异无统计学意义(P均>0.05)。sTRAILR1分别与sTRAIL、sTRAILR4的蛋白浓度呈显著正相关(n=102,r分别为0.236和0.409,P均<0.01)。 【结论】 毛细支气管炎和急性上呼吸道感染患儿血清中可溶性TRAIL及其受体TRAILR1和TRAILR4的表达增加,它们可能参与了呼吸道感染的炎症过程,TRAIL可能主要通过TRAILR1起作用,它们的表达水平增高可能被视为病情活动的指标之一。

Expressions of serum soluble tumor necrosis factor related apoptosis inducing ligand and its receptor 1 and 4 in patients with bronchiolitis and acute upper respiratory infection

【 Objective 】 To investigate the potential roles of tumor necrosis factor related apoptosis inducing ligand(TRAIL) and its soluble receptors(sTRAILR1and sTRAILR4) in the pathogenesis of respiratory tract infections,the concentrations of sTRAIL,sTRAILR1and sTRAILR4proteins were determined.【 Methods 】 Concentrations of sTRAIL and sTRAILR1and sTRAILR4protein were detected by ELISA,in patients with bronchiolitis and acute upper respiratory infection and individuals(named bronchiolitis group,AURI group,control group).【 Results 】 The concentrations of sTRAIL,sTRAILR1and sTRAILR4protein were significantly higher in bronchiolitis group(85±38),(71±19),(167±97) pg / mL,respectively and in AURI group(78±28),(61±15),(139±72) pg / mL than those in control group(55±19),(45±14),(56 ±38) pg / mL,respectively(Pall<0.01).Data also showed that the concentration of sTRAILR1protein was markedly higher in bronchiolitis group than that in AURI group(P all<0.05).While no significance were observed of sTRAIL and sTRAILR4expressions between the patients with bronchiolitis group and in AURI group(P>0.05).Furthermore,levels of sTRAILR1was highly correlated to sTRAIL and sTRAILR4(n=102,r=0.236and 0.409respectively,all P<0.01).【 Conclusion 】 Levels of sTRAIL,sTRAILR1and sTRAILR4were elevated in bronchiolitis and AURI which could be applied as an effective targets of patient’s condition judgment.Our study indicated TRAIL and its receptors may involved in the pathogenesis of bronchiolitis and AURI inflammation.