脓毒症对大鼠海马区caspase-3表达的影响

目的 探讨脓毒症对大鼠海马区神经元的凋亡蛋白酶caspase-3表达的影响。 方法 80只30日龄健康雄性Wistar大鼠，随机分为盲肠结扎穿孔术（CLP） 组（n=50）和对照组（n=30）。CLP组采用CLP建立脓毒症模型，对照组行假手术不造成脓毒症模型。于手术后6、12、24 h，CLP组和对照组分别取10只大鼠，5只做神经行为学评分，另外5只处死取脑，采用蛋白免疫印迹法观察caspase-3的表达，术后24 h，两组各取3只大鼠，采用免疫荧光检测caspase-3的表达。 结果 对照组大鼠大脑海马区仅微量表达caspase-3，神经行为学评分较高。CLP组大鼠caspase-3的表达量于CLP后6 h就开始升高，24 h达高峰，均明显高于对照组（P＜0.05），大鼠的神经行为学评分在CLP后6 h开始降低，并随着时间逐渐下降，均明显低于对照组（P＜0.05）。 结论 脓毒症脑损伤时大鼠的神经行为学评分降低，海马区神经元caspase-3表达上调，并随时间变化而波动。

Influence of Sepsis on the Expression of Apoptotic Protease Caspase-3 in Hippocampus of Rats

Objective To investigate the influence of sepsis on the expression of apoptotic protease caspase-3 in hippocampus neurons of rats. Methods Models of rats with sepsis were established by the cecal ligation and puncture （CLP） method. Eighty 30-day-old male Wistar rats were randomly divided into CLP group （n=50） and control group （n=30）. In the CLP group, only CLP was performed on the rats. Ten rats in the CLP group and another 10 in the control group were taken at 6, 12, and 24 hours after operation, respectively. Five of them in each group were taken for neurobehavioral score, and the other five were killed and their brains were removed. Then the Western blot and immunohistochemistry staining were used to detect the expression changes of apoptosis protein caspase-3. Results In the control group, there were very low expression of apoptotic protease caspase-3 and high scores of neurological behavior. In the CLP group, the expression of apoptotic protease caspase-3 started to increase at the 6th hour, and reached the peak at the 24th hour after CLP, both of which were significantly higher than the control group （P 〈 0.05）. The scores of neurological behavior of the CLP group began to decline at 6h after CLP, and decreased gradually along with the time, and the scores were significantly lower at various time points after CLP in the CLP group than those in the control group （P 〈 0.05）. Conclusion The scores of neurological behavior decrease and the expression of apoptosis protease caspase-3 increase in the rat hippocampus with sepsis, and fluctuate with time change.