苹果多酚对卡介苗联合脂多糖所致小鼠免疫性肝损伤的保护作用

目的:观察苹果多酚(Apple Polyphenols,AP)对小鼠免疫性肝损伤的保护作用。方法:采用卡介苗联合脂多糖制备小鼠免疫性肝损伤模型。研究AP对小鼠肝指数和脾指数,血清中谷草转氨酶(Aspartate Transaminase,AST)和谷丙转氨酶(Alanine Transaminase,ALT)活性,血清总蛋白(Total Protein,TP),白蛋白(Albumin,Alb)和白蛋白/球蛋白(A/G)含量的改善作用,同时采用HE染色方法观察AP对免疫性肝损伤小鼠肝脏病理改变的保护作用。结果:AP能明显抑制免疫性肝损伤模型小鼠异常升高的肝指数和脾指数,降低血清中AST活性,不同程度地升高血清中TP和Alb的含量,同时对肝组织病理异常变化也有显著的改善作用。结论:AP对卡介苗联合脂多糖所致的小鼠免疫性肝损伤具有保护作用。

Hepatoprotective Role of Apple Polyphenols against Immunological Liver Injury in Mice

Objective：To observe hepatoprotective role of apple polyphenols （AP,Appjfnol ）on immunological liver injury in mice. Methods：An immunological liver injury model in mice was established by intravenous injection of Bacillus Calmette-Guerin （BCG）and lipopolysaccharide （LPS）.The liver index and spleen index,the activities of serum aspartate aminotransferase （AST）and alanine ami-notransferase （ALT）,the levels of serum total protein （TP）,albumin （Alb）and albumin/globulin （A /G）were detected.Histopatho-logic change was also investigated by HE staining.Results：AP inhibited the increases of liver index and spleen index,prevented the in-crease of serum AST activity and the decrease of serum TP and Alb levels.Histopathological abnormalities were also improved after AP administration.Conclusion：AP played a significant hepatoprotective role against BCG and LPS induced immunological liver injury in mice.