Monoclonal antibodies reactive with specific amino acid sequences of the 126K protein of tobacco mosaic virus

Summary A structure/function study has been initiated for the ³⁴ bacteriophage proteins involved in lysogeny inSalmonella newington. Hydroxylamine and nitrosoguanidine mutagenesis of a wild type ³⁴ phage was used to generate clear plaque variants. Complementation analysis was used to define four genes involved in the phage lysogenic pathway. A relative mapping order has been established. In addition, a virulent mutant, ³⁴vir82, which defines a repressor binding site has been isolated.Dedicated to the memory of Professor Harrison Hatch Echols of the Department of Molecular and Cell Biology of the University of California at Berkeley.