|
|||||
|
|
| 麝香酮在大鼠肠灌注液中GC-MS/MS 测定方法及其大鼠肠吸收动力学特征 | |
| 引用本文： | 邹亮,林俊芝,王战国,许丽佳,王平,赵钢,罗杰英.麝香酮在大鼠肠灌注液中GC-MS/MS 测定方法及其大鼠肠吸收动力学特征[J].中国中药杂志,2012,37(16):2456-2460. |
| 作者姓名： | 邹亮 林俊芝 王战国 许丽佳 王平 赵钢 罗杰英 |
| 作者单位： | 1. 成都大学生物产业学院,四川成都,610106 2. 成都中医药大学药学院,四川成都,610075 3. 四川大学生命科学学院,四川成都,610065 4. 湖南中医药大学药学院,湖南长沙,410007 |
| 基金项目： | 国家自然科学基金项目(81001650) |
| 摘 要： | 目的:建立麝香酮在大鼠肠灌注液中的GC-MS/MS测定方法,研究麝香酮的大鼠小肠吸收动力学特征。方法:采用GC-MS/MS测定大鼠肠循环液中的麝香酮含量;采用大鼠原位肠循环灌注法进行麝香酮吸收动力学研究。结果:麝香酮在各小肠段均有较好吸收;其吸收速度常数(Ka)和每小时吸收率(A),十二指肠>空肠(P<0.05),十二指肠>回肠(P<0.01)。麝香酮在小肠段的Ka,A和吸收半衰期T1/2分别为0.990 h-1,43.58%,0.705 h。结论:麝香酮在整个肠道内均有较好吸收,十二指肠Ka,T1/2优于空肠(P<0.05),十二指肠Ka,T1/2,A显著优于回肠(P<0.01,P<0.01,P<0.05),空肠与回肠无显著差异。 |
| 关 键 词： | 麝香酮 吸收动力学 原位肠灌注 GC-MS/MS |
| 收稿时间： | 2012/3/17 0:00:00 |
Determination method of muscone in rat intestinal perfusate by GC-MS/MS and its intestinal absorption kinetic characteristics in rats |
|
| ZOU Liang,LIN Junzhi,WANG Zhanguo,XU Liji,WANG Ping,ZHAO Gang and LUO Jieying.Determination method of muscone in rat intestinal perfusate by GC-MS/MS and its intestinal absorption kinetic characteristics in rats[J].China Journal of Chinese Materia Medica,2012,37(16):2456-2460. | |
| Authors: | ZOU Liang LIN Junzhi WANG Zhanguo XU Liji WANG Ping ZHAO Gang and LUO Jieying |
| Institution: | College of Biological Industry, Chengdu University, Chengdu 610106, China;College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;College of Life Sciences, Sichuan University, Chengdu 610065, China;College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China;College of Biological Industry, Chengdu University, Chengdu 610106, China;College of Pharmacy, Hunan University of Traditional Chinese Medicine, Changsha 410007, China |
| Abstract: | Objective : To establish the method for determining muscone in rat intestinal perfusate by GC-MS/MS and study its intestinal absorption kinetic characteristics in rats. Method : The GC-MS/MS method was used to determine the content of muscone in rat intestinal circulation fluid. In situ intestinal circulation perfusion was adopted to study absorption kinetics of muscone in rats. Result : Muscone was proved to be well absorbed in each section of small intestine. Its absorption rate constants (Ka) and the absorption rate (A) in the rat intestine showed duodenum>jejunum (P<0.05), duodenum>ileum (P<0.01). Its Ka, A and t1/2 in rat small intestine was 0.990 h-1, 43.58% and 0.705 h, respectively. Conclusion : Muscone was well absorbed in each intestinal section, with duodenum better than jejunum (Ka, T1/2, P<0.05) significantly better than ileum (Ka, T1/2, P<0.01; A, P<0.05). There is no obvious statistical difference between jejunum and ileum. |
| Keywords: | muscone absorption kinetics in situ intestinal circulation perfusion GC-MS/MS |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |