HPLC同时测定栀子中8个环烯醚萜苷类成分的含量

目的:建立HPLC同时测定栀子中8个环烯醚萜苷类成分的含量测定方法。方法:采用Kromasil C18色谱柱(4.6mm×250 mm,5μm),流动相为乙腈-水-三氟乙酸(6∶94∶0.05),流速1.0 mL.min-1,检测波长238 nm,柱温40℃。结果:栀子苷,栀子新苷,山栀苷,栀子苷酸,去乙酰车叶草苷酸甲酯,羟异栀子苷,鸡矢藤次苷甲酯和京尼平龙胆二糖苷的线性范围(μg)和加样回收率分别为1.503 6～15.036(99.6%),0.042 56～0.425 6(100.6%),0.103 8～1.038(101.2%),0.009 92～0.099 2(99.5%),0.023 32～0.233 2(100.3%),0.412 8～4.128(98.7%),0.020 40～0.204 0(99.8%)和0.465 6～4.656(100.1%)。结论:该方法分离良好,简便准确,重复性好,可更好地为栀子药材的质量控制提供依据。

Simultaneous determination of eight iridoid glycosides in Gardeniae Fructus by HPLC

Objective: To establish an HPLC method for simultaneous determination of eight iridiods in Gardeniae Fructus.Method: Kromasil C18 column(4.6 mm×250 mm,5 μm) was adopted,with acetonitrile-water-trifluoroacetic acid(6∶94∶0.05) as the mobile phase at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 238 nm,and the column temperature was 40 ℃.Result: The linear ranges of geniposide,gardoside,shanzhiside,geniposidic acid,deacetyl asperulosidic acid methyl ester,gardenoside,scandoside methyl ester,and genipin gentiobioside were 1.503 6-15.03 6,0.042 56-0.425 6,0.103 8-1.038,0.009 92-0.099 2,0.023 32-0.233 2,0.412 8-4.128,0.020 40-0.204 0 and 0.465 6-4.656 μg,respectively.Their average recoveries were 99.6%,100.6%,101.2%,99.5%,100.3%,98.7%,99.8% and 100.1%,respectively.Conclusion: The method shows good separation and it is so simple,accurate and highly repeatable that it can be used for providing basis for quality control of Gardeniae Fructus.