当归补血汤多糖对隐孢子虫感染小鼠肠道局部免疫功能的影响

目的：探讨当归补血汤多糖对免疫低下小鼠感染隐孢子虫后的免疫调节作用。方法：用微小隐孢子虫卵囊（CSO）感染免疫抑制BALB／c小鼠，建立隐孢子虫感染的动物模型，再给该模型小鼠灌服当归补血汤多糖8天后，ELISA法检测小鼠肠道局部IL-2、IL-4及SIgA的水平，HE染色检查肠道病理学改变。同时设模型组和正常组作对照。结果：当归补血汤多糖能促进隐孢子虫感染小鼠肠道IL-2、IL-4、SIgA的分泌；肠道粘膜病理改变明显好转。结论：当归补血汤多糖对小鼠隐孢子虫感染具有免疫增强作用。

THE EFFECTS OF DANGGUI BUXUE DECOCTION POLYSACCHA RIDES ON THE LOCAL IMMUNLOGICAL FUNCTION OF IMMUNOSUPPRESSED MICE INFECTED WITH CRYPTOSPORIDIUM OOCYST

Objective：To investigate the effect of immunomodulation of Danggui Buxue decoction polysaccharides on the local immunological function of immunosuppressed mice infected with cryptosporidium oocyst. Methods： Mouse models were induced with oral inoculating cryptospridium parvum oocysts after being immusuppressed with Dexamethasone （DEX）. And then the mice were perfused with Danggui Buxue decoction for 8 days, in the same time the model group and normal group as control. IL were detect with ELISA assay, and intestinal -2 and IL- 4 in intestines homogenate and SIgA. of intestines tissue pathological change were detected by HE staining. Results： Danggui Buxue decoction polysaccharides can promote local IL -2, IL -4 and SIgA secreting. Intestinal pathological changes obviously improved. Conelusions：Danggui Buxue decoction polysaccharides effect on immunosuppressed mice infected with cryptosporidiu oocyst.