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| Bcl-xl和Bcl-2基因在人和兔心肌细胞中的同源性研究 | |
| 引用本文： | 马中富,马虹,高劲松,何建桂,叶任高,罗斌,何蕴韶. Bcl-xl和Bcl-2基因在人和兔心肌细胞中的同源性研究[J]. 中国急救医学, 2000, 20(10): 570-572 |
| 作者姓名： | 马中富 马虹 高劲松 何建桂 叶任高 罗斌 何蕴韶 |
| 作者单位： | 1. 中山医科大学附属第一医院急诊科,广州,510080 2. 中山医科大学附属第一医院心内科,广州,510080 3. 中山医科大学附属第一医院肾内科,广州,510080 4. 中山医科大学法医教研室 5. 中山医科大学达安基因诊断中心 |
| 基金项目： | 卫生部基金!资助项目 ( 1996 -1-116 ) |
| 摘 要： | 目的 探讨兔和人的有核细胞中Ｂｃｌ－ｘｌ和Ｂｃｌ－２基因的同源性。方法 用人的Ｂｃｌ－ｘｌ和Ｂｃｌ－２基因序列来扩增兔心肌细胞的ＤＮＡ，纯化其扩增产物，然后进行测序，再测定人与兔心肌细胞内Ｂｃｌ－ｘｌ和Ｂｃｌ－２基因的同源性。结果 兔心肌细胞内Ｂｃｌ－ｘｌ基因的扩增产物长度为１６２个碱基，与人类Ｂｃｌ－ｘｌ基因的碱基序列进行比较，在扩增的１５９个碱基片段中有１５１个碱基相同，其同源性为９５．０％； |
| 关 键 词： | Bcl－xl基因 Bcl－2基因 同源性 心肌细胞 |
| 文章编号： | ］ |
Study of Bcl-xl and Bcl-2 gene homogenous in rabbit cardiac myocytes and human karyotes |
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| MA Zhoug-fu ,MA Hong,GAO Jin-song ,et al.. Study of Bcl-xl and Bcl-2 gene homogenous in rabbit cardiac myocytes and human karyotes[J]. Chinese Journal of Critical Care Medicine, 2000, 20(10): 570-572 | |
| Authors: | MA Zhoug-fu MA Hong GAO Jin-song et al. |
| Affiliation: | MA Zhoug-fu 1,MA Hong,2GAO Jin-song 2,et al. 1 Emergency Department,the First Affiliated Hospital,Sun Yat-Sen University of Medical Sciences,Guangzhou 510080,China [ |
| Abstract: | Objective To investigate Bcl-xl and Bcl-2 gene homogeneity in rabbit cardiac myocytes and human karyotes.Methods DNA in rabbit cardiac myocytes were amplificated by using human Bcl-xl gene and Bcl-2 gene sequence.The amplificated products of DNA in rabbit myocytes were purificated before sequenced.The homogenous of Bcl-xl gene and Bcl-2 gene in rabbit myocardial cells and human karyotes were analysed.Results The length of the amplificated products of Bcl-xl gene in rabbit myocardial cells was 162 bases.The 95.0% of Bcl-xl gene in rabbit myocytes was homogeneous with human Bcl-xl gene in 159 bases sequences.And the length of the amplificated products of Bcl-2 gene in rabbit myocardial cells was 288 bases.The 88.4% of Bcl-2 gene in rabbit myocytes was homogenous with human Bcl-2 gene in 199 bases.Conclusions There were good homogeneities of Bcl-xl gene and Bcl-2 gene in between rabbit myocytes and human karyotes.Human Bcl-xl and Bcl-2 gene sequence,therefore,is completely used to amplificate both genes in rabbit cardiac myocytes when studying,which offerefore,basic theory for further study. [ |
| Keywords: | Bcl-xl Bcl-2 Rabbit Human Homogeneity |
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