高效液相色谱法同时测定荷丹片中荷叶碱丹参酮ⅡA补骨脂素和异补骨脂素的含量

目的 采用高效液相色谱法同时测定荷丹片中荷叶碱、丹参酮ⅡA、补骨脂素和异补骨脂素的含量.方法 采用50%乙醇超声提取,在色谱柱Agilent SB-Aq C18(4.6 mmx250 mm,5μm)上进行分离,柱温为25%,流动相:A相为水-乙腈--三乙胺--冰醋酸(355:30:9:3.9);B相:乙腈,梯度洗脱:0...

High Performance Liquid Chromatography Determination of Nuciferine Tanshinone Psoralen and Isopsoralen in Compound Hedan Pills

Objective To develop an high performance liquid chromatography method for simultaneous determination of nuciferine,tanshinone ⅡA,psoralen and isopsoralen in compound Hedan pills.Methods The determination was carried out over an Agilent SB-Aq C18（4.6 mm ×250 mm,5 μm） at the temperature 25℃.The mobile phase consisted of（A） H2O-ACN-TEA-HAc（355∶30∶9∶3.9）（B） ACN solution with the gradient elution 0-11 min,30%B-20%B,11-18 min,20%B-20%B,18-30 min,20%B-70%B,30-40 min,70%B-70%B.The flow rate was 1 ml/min,and the detection wavelength was set at 245 nm（detecting psoralen and isopoaln） and 322 nm（detecting nuciferine and tanshinone ⅡA）.Results The method showed a good linear relationship within the range of 13.028-103.28 μg/ml for nuciferine（r=0.9999）,1.124-11.24 μg/ml for tanshinone ⅡA（r=0.9999）,6.630-66.30 μg/ml for psoralen（r=0.9999） and 6.385-63.85 μg/ml for isopsoralen（r=0.9999）.The average recovery was 95.13%（RSD was 1.07%）for nuciferine,100.14%（RSD was 1.84%） for tanshinone ⅡA,99.76%（RSD was 1.80%） for psoralen and 101.41%（RSD was 1.86%） for isopsoralen.The sample solution was stable within 12 h.Conclusion The method is simple,accurate,sensitive,and reproducible to test major ingredients in Hedan pills.