走马胎中的三萜皂苷类成分及其体外抗肿瘤活性研究

目的 研究走马胎根茎的三萜皂苷类成分及其体外抗肿瘤活性.方法 采用溶剂萃取和柱色谱方法分离纯化,根据理化性质和波谱学数据鉴定化合物结构.采用MTT法测试三萜皂苷化合物的抗肿瘤活性.结果 分离得到了7个三萜皂苷类化合物,包括3β-O- β-D-吡喃木糖基-(1→2)-β-D-吡喃葡萄糖基-(1→4)-α-L-吡喃阿拉伯糖...

Triterpenoid Saponins and the Antitumor Activity from the Rhizome of Ardisia Gigantifolia Stapf

Objective To study triterpenoid saponins and the antitumor activity of the 60% ethanol extract of the rhizome of Ardisia gigantifolia Stapf.Methods The chemical components were isolated by solvent extraction and column chromatography and their structures were identified on the basis of physical-chemical constants and spectral data.The antitumor activities of the compounds were screened by MTT methods.Results Seven triterpenoid saponins were isolated and identified as 3β-O-{β-D-xylopyranosyl-（1→2）-β-D-glucopyr-anosyl-（1→4）-α-L-arabinopyranosyl}-3β-hydroxy-13β,28-epoxy-16β-hydroxy-30-al（1）;cyclamiretin A 3β-O-α-L-rhamnopyranosyl（1→3）-β-D-xylopyranosyl-（1→2）]-β-D-glucopyranosyl-（1→4）-β-D-glucopyranosyl（1→2）]-α-L-arabinopyranoside（2）;lysikoianoside（3）;cyclamiretin A 3β-O-α-L-rhamnopyranosyl-（1→3）-β-D-glucopyranosyl-（1→3）-β-D-xylopyranosyl-（1→2）]-β-D-glucopyranosyl-（1→4）-β-D-glucopyranosyl-（1→2）]-α-L-arabinopyranoside（4）,3β-O-{α-L-rhamnopyranosyl-（1→3）-β-D-xylopyranosyl-（1→2）]-β-D-galactopyranosyl-（1→4）-β-D-6-O-acetyl-glucopranosyl-（1→2）]-α-L-arabinopyranoside}-cyclamiretin A（5）,Ardisiacri-dpin A（6） and 3β-o-{α-L-rhamnopyranosyl-（1→3）-β-D-xylopyranose-（1→2）]-β-D-glucopyranosyl-（1→4）-α-L-arabinopyranosyl}-3β-hydroxy-13β,28-epoxyoleanan-16-oxo-30-al（7）.Conclusion Compound 1 and 3 were obtained from this plant and 7 from this genus for the first time.Among the compounds,No.2 showed cytotoxic activity of 0.29,9.99 and 2.03 μg/ml（IC50） in BCG-823,EJ and Hepg2 cells respectively.Compound 3 showed selective cytotoxic activity to EJ cells（IC50 7.20 μg/ml）.Compound 7 showed cytotoxic activity of 8.53 μg/ml（IC50） in Hepg2 cells.