高效液相色谱法同时测定人血清中卡铂、假尿苷含量

 目的建立一种同时检测人血清中卡铂、假尿苷含量的高效液相色谱方法。方法色谱条件:Spherigel-C₁₈色谱柱;流动相:含0.5%乙腈的0.02 mol·L^-1KH₂PO₄缓冲液;流速:0.95 mL·min^-1;UV:230 nm。结果本法卡铂的线性范围为1～20mg·L^-1,相关系数r=0.990 2,平均回收率96%,最低检测浓度0.5 mg·L^-1;假尿苷的线性范围为1～16 mg·L^-1,相关系数r= 0.998 6;平均回收率97%,最低检测浓度0.3 mg·L^-1。结论本法具有样品处理简单、稳定性高等特点,经临床验证具有较好的实用性且易于推广应用。

Determination of carboplatin and pseudouridine in the human serum by HPLC

OBJECTIVE To establish a method for the determination of carboplatin and pseudouridine in human serum.METHODS The concentration of carboplatin and pseudouridine was assayed on a Spherigel-C₁₈ column with a mobile phase consisting of acetonitrile-0.02 mol·L^-1 KH₂PO₄(5:1 000)at a flow rate of 0.95 mL·min^-1 and detected at 230 nm. RESULTS The calibration curve of carboplatin was linear over the range of 1～20 mg·L^-1 (r= 0.990 2) .the average recovery of carboplatin was 97% . The limit of determination was 0.5 mg·L^-1.The calibration curve of pseudouridine was linear over the range of 1～16 mg·L^-1(r = 0.998 6). The average recovery of pseudouridine was 97% . The limit of determination was 0.3 mg·L^-1. CONCLUSION This method is simple and highly stabile. Clinical application demonstrated that this method was practical.