| 机械拉伸下人牙周膜成纤维细胞增殖动力学变化的研究 |
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| 引用本文： | 韩文利,陈新民. 机械拉伸下人牙周膜成纤维细胞增殖动力学变化的研究[J]. 四川大学学报(医学版), 2000, 31(2): 149-151,168 |
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| 作者姓名： | 韩文利 陈新民 |
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| 作者单位： | 1. 卫生部口腔医疗社区保健网蓝钻石口腔诊所
2. 华西医科大学口腔医学院,口腔修复教研室,成都,610041 |
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| 基金项目： | 四川省科技厅资助项目，， |
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| 摘 要： | 为了研究拉伸应变对人牙周膜成纤维细胞DNA合成的影响,采用流式细胞术对拉伸应变下的细胞增殖动力学变化进行分析.结果:在0.5%～8%拉伸应变范围内,人牙周膜成纤维细胞受力2小时即显示出较高的增殖活性,6小时达高峰,其中以2%拉伸应变作用最为明显,随时间延长,增殖指数下降.提示拉伸应变可有效促进人牙周膜成纤维细胞DNA合成.
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| 关 键 词： | 机械拉伸 DNA合成 人牙周膜成纤维细胞 流式细胞术 |
The Effects of Mechanical Stretching on DNA Synthesis of Human Periodontal Ligament Fibroblasts |
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| Han Wenli,Chen Xinmin. The Effects of Mechanical Stretching on DNA Synthesis of Human Periodontal Ligament Fibroblasts[J]. Journal of Sichuan University. Medical science edition, 2000, 31(2): 149-151,168 |
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| Authors: | Han Wenli Chen Xinmin |
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| Abstract: | This study was aimed at the effects of mechanical stretching on DNA synthesis of human periodontal ligament fibroblasts(PDLF). Flow cytometry(FCM) was used to examine the proliferative activities of PDLF grown on the flexible culture dishes subjected to mechanical stretching. The results showed that in the range of 0. 5%-8%, mechanical stretching could stimulate DNA synthesis 2 hours after the cells had been subjected to stretches. DNA synthesis reached the peak in the 6 hours. The effect of 2% stretching was more significant. As time went by, the mitogenic effect of stretch was decreased. These findings suggest that mechanical stretching can stimulate DNA synthesis of PDLF. |
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| Keywords: | Mechanical stretching DNA synthesis Periodontal ligament fibroblast Flow cytometry |
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