荧光原位杂交技术检测宫颈病变中C-MYC基因的表达及其意义

目的:探讨c-myc原癌基因在宫颈病变中的表达及其临床意义。方法:筛选114例宫颈细胞涂片,按最终病理学结果分组,其中正常宫颈20例,CINⅠ20例,CINⅡ25例、CINⅢ25例、宫颈癌24例;同时选取上述病例的宫颈病理切片,均用FISH方法检测c-myc基因的表达情况。结果:①C-myc基因在不同细胞学分级中即ASCUS、LSIL、HSIL和SCC宫颈脱落细胞中的阳性表达率为0.0%、20.8%、38.9%、61.2%、95.2%,各组间差异有统计学意义(P<0.01)。②C-myc基因在不同病理学分级中即正常宫颈、CINⅠ、CINⅡ、CINⅢ及宫颈癌脱落细胞中的阳性表达率分别为0.0%、10.0%、44.0%、72.0%、90.2%,其组间差异有统计学意义(P<0.01)。③在宫颈病理切片组织中C-myc基因阳性表达率在正常宫颈、CINⅠ、CINⅡ、CINⅢ及宫颈癌组织中依次为0.0%、20.0%、56.0%、80.0%、95.0%,组间差异有统计学意义(P<0.05)。结论:c-myc基因在各级CIN和宫颈癌的脱落细胞及组织中均有异常扩增,其扩增率随着宫颈病变程度增加而增加,提示c-myc基因异常扩增在宫颈病变筛查中具有一定的价值和意义。

Expression and significance of c-myc gene in cervical lesions detected by fluorescence in situ hybridization technique

Objective:To explore the expression and clinical significance of c-myc oncogene in cervical lesions.Methods:One hundred and fourteen cervical cytological smears were selected and divided into different groups according to the final pathological results,20 cases with normal cervical tissue,20 cases with cervical intraepithelial neoplasia(CIN) Ⅰ,25 cases with CIN Ⅱ,25 cases with CIN Ⅲ,and 24 cases with cervical cancer were included.The cervical pathological smears of the above-mentioned cases were obtained,fluorescence in situ hybridization(FISH) technique was used to detect the expression level of c-myc gene.Results:The positive expression rates of c-myc gene in cervical exfoliated cells specimens of atypical squamous cells of undetermined significance(ASCUS) group,low-grade squamous intraepithelial lesion(LSIL) group,high-grade squamous intraepithelial lesion(HSIL) group,and squamous cell carcinoma(SCC) group were 20.8%,38.9%,61.2%,and 95.2%,respectively,there was significant difference among different groups(P<0.01).The positive expression rates of c-myc gene in cervical exfoliated cells specimens of normal cervical tissue group,CIN Ⅰ group,CIN Ⅱ group,CIN Ⅲ group,and cervical cancer group were 0.0%,10.0%,44.0%,72.0%,and 90.2%,respectively,there was significant difference among different groups(P<0.01).The positive expression rates of c-myc gene in cervical pathological smears of normal cervical tissue group,CIN Ⅰ group,CIN Ⅱ group,CIN Ⅲ group,and cervical cancer group were 0.0%,20.0%,56.0%,80.0%,and 95.0%,respectively,there was significant difference among different groups(P<0.05).Conclusion:c-myc gene amplifies in cervical exfoliated cells and cervical pathological smears of CIN Ⅰ,CIN Ⅱ,CIN Ⅲ,and cervical cancer unusually,the amplification rate increases with the aggravation of cervical lesions,which indicates that the abnormal amplification of c-myc gene has a certain value and significance in screening of cervical lesions.