内皮抑素在子宫内膜异位大鼠模型致低妊娠功能的治疗作用

目的:建立大鼠子宫内膜异位(EMT)模型,观察内异症所致低妊娠状态,并探讨内皮抑素(恩度)的治疗作用。方法:自体移植术建立SD大鼠EMT模型,造模成功后随机分为模型组、达那唑组、恩度组,8只行假手术。术后4周开始用药,用药14天,各组随机选半数大鼠处死,取血清检测FSH、LH、E2、P,取异位灶切片观察组织大体情况,免疫组化检测VEGF表达。余下鼠雌雄合笼观察妊娠状态。结果:32只大鼠造模,成功28只,假手术组、达那唑组、恩度组VEGF表达低于模型组(P<0.05),血清FSH、LH、E2、P水平前3组与模型组比较差异有统计学意义(P<0.05),模型组处于低妊娠状态,恩度组与达那唑组受孕情况比较差异有统计学意义(P<0.05)。结论:SD大鼠子宫内膜异位症模型建立成模率高,能模拟内异症所致低妊娠状态,恩度对内异症所致低妊娠功能有一定的治疗作用,且不良反应小。

Curative effect of endostatin in rat models with endometriosis-induced low gestational function

Objective:To establish the rat model of endometriosis,observe the endometriosis-induced low gestational status,explore the curative effect of endostatin(Endostar).Methods:The SD rat models of endometriosis were established by autologous transplantation method,then the rats were randomly divided into model group,Danazol group,and Endostar group,8 rats underwent sham operation.The rats were treated with drug at four weeks after operation for fourteen days,half of rats from each group were selected at random and then killed,the serum samples were obtained to detect the levels of follicle stimulating hormone(FSH),luteinizing hormone(LH),estradiol,and progesterone;the sections of ectopic pregnancy focuses were obtained to observe the general situation,immunohistochemistry was used to detect the expression of vascular endothelial growth factor(VEGF).The remaining rats were caged together with male rats to observe the gestational function.Results:A total of 32 rats were operated,and 28 rats succeeded.The expression levels of VEGF in sham operation group,Danazol group,and Endostar group were significantly lower than that in model group(P<0.05).There was significant difference in the serum levels of FSH,LH,estradiol,and progesterone between the first three groups and model group(P<0.05).The rats in model group were in low gestational status.There was significant difference in conception situation between Endostar group and Danazol group(P<0.05).Conclusion:The success rate of SD rat models of endometriosis is high,the rat models can simulate endometriosis-induced low gestational status,Endostar has a certain therapeutic effect for endometriosis-induced low gestational function,and it has less adverse reactions.