| 血清HBV-DNA检测的临床价值 |
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| 引用本文： | 陈永良,张仲华,黄永章,陈刚.血清HBV-DNA检测的临床价值[J].郧阳医学院学报,1994(3). |
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| 作者姓名： | 陈永良 张仲华 黄永章 陈刚 |
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| 作者单位： | 郧阳医学院医学实验中心，上海医科大学免疫学教研室 |
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| 摘 要： | 选用分子斑点杂交法和多聚酶链反应（ＰＣＲ）观察１０７例疑为乙型肝炎患者ＨＢＶ－ＤＮＡ检出情况，分析ＨＢＶ－ＤＮＡ与ＨＢＶ血清抗原抗体标记物检出的关系，初步比较目前采用的这两种方去，认为分子斑点杂交法在应用中优于ＰＣＲ，值得推广。
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| 关 键 词： | 乙型病毒性肝炎，脱氧核糖核酸，分子杂交，多聚酶链反应 |
Clinical Uses of Detection of Serum Hepatitis B Virus DNA Using Dot-hybridization and Polymerase Chain Reaction |
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| Chcn Yongliang, et al.Clinical Uses of Detection of Serum Hepatitis B Virus DNA Using Dot-hybridization and Polymerase Chain Reaction[J].Journal of Yunyang Medical College,1994(3). |
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| Authors: | Chcn Yongliang |
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| Abstract: | Polymerase chain reaction(PCR) assay and dot-hybridization test were used in sera of 107 patients to detect hepatitis B virus(HBV) DNA. The results of two methods were similer. Detection of HBV-DNA in serum was obviously relative to patients with positive for HBeAg or HBeAb, if HBsAg and HBcAb was positive in dot-hybridization test; 3 patients were positive for HBV-DNA in 4 cases who were negative for HBsAg,HBeAg,HBeAb and HBcAb.These data showed that dot-hyridization test was useful in detection of serum HBV-DNA. |
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| Keywords: | viral hepatitis B DNA dot-hybridization PCR |
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