RNAi抑制XJ-160病毒复制的研究

目的通过建立RNA干扰(RNA interference，RNAi)抑制XJ-160病毒复制的模型，在序列特异性、位置效应和量效关系等方面，探讨RNAi对XJ-160病毒复制的影响，为进一步研究RNAi的抗病毒作用和机制奠定基础。方法按照siRNA(short interferencing RNA)的设计要求合成XJ-160病毒特异siRNA，脂质体法转染BHK-21细胞后感染XJ-160病毒，通过测定病毒滴度、蛋白表达量及XJ-160病毒：RNA合成研究siRNA对XJ-160病毒复制的抑制。结果成功建立了RNAi抑制XJ-160病毒复制的模型，探讨了RNAi抑制该病毒复制作用的特点。结论外源导入的siRNA能够抑制XJ-160病毒的复制，并且这种抑制作用具有明显的序列特异性、位置效应和存在量效关系等特点；siRNA是通过降解病毒RNA实现抑制病毒复制作用的。

Inhibition of XJ-160 virus replication by RNAi

Objective By construct the model of RNAi-mediated inhibition of XJ-160 virus replication to study RNAi-mediated inhibition of XJ-160 virus replication on aspect of sequence, positional and dose dependent, to further research on effect and mechanism of RNAi on antiviral effects. Methods Synthesized the XJ-160 virus special short interferencing RNA (siRNA) according to the requiring of siRNA design. siRNA was transfected into BHK-21 cells via liposomes, then infected the BHK-21 cells by XJ-160 virus. Through detecting the titer, protein expression and RNA synthesizing of XJ-160 virus, research on siRNA -mediated inhibition of XJ-160 virus replication. Results Constructed the model of RNAi-mediated inhibition of XJ-160 virus replication successfully, and discussed the character of it. Conclusion siRNA can inhibit the replication of XJ-160 virus. It has sequence, positional and dose dependent. And it is carried out by digested the virus RNA.